Publication Date:
1996-08-09
Description:
In axons, cytoskeletal constituents move by slow transport. However, it remains controversial whether axonal neurofilaments are dynamic structures in which only subunits are transported or whether filaments assemble in the proximal axon and are transported intact as polymers to the axon terminus. To investigate the form neurofilament proteins take during transport, neurons of transgenic mice lacking axonal neurofilaments were infected with a recombinant adenoviral vector encoding epitope-tagged neurofilament M. Confocal and electron microscopy revealed that the virally encoded neurofilament M was transported in unpolymerized form along axonal microtubules. Thus, neurofilament proteins are probably transported as subunits or small oligomers along microtubules, which are major routes for slow axonal transport.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Terada, S -- Nakata, T -- Peterson, A C -- Hirokawa, N -- New York, N.Y. -- Science. 1996 Aug 9;273(5276):784-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Brain Research, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8670416" target="_blank"〉PubMed〈/a〉
Keywords:
Adenoviridae/genetics
;
Animals
;
*Axonal Transport
;
Axons/chemistry/*metabolism/ultrastructure
;
Ganglia, Spinal/virology
;
Genetic Vectors
;
Mice
;
Mice, Transgenic
;
Microscopy, Confocal
;
Microscopy, Immunoelectron
;
Microtubules/*metabolism
;
Neurofilament Proteins/analysis/*metabolism
;
Proto-Oncogene Proteins c-myc/genetics/metabolism
;
Recombinant Proteins/metabolism
;
Sciatic Nerve/chemistry/ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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