Publication Date:
2002-06-18
Description:
Fanconi anemia (FA) is a rare autosomal recessive cancer susceptibility disorder characterized by cellular hypersensitivity to mitomycin C (MMC). Six FA genes have been cloned, but the gene or genes corresponding to FA subtypes B and D1 remain unidentified. Here we show that cell lines derived from FA-B and FA-D1 patients have biallelic mutations in BRCA2 and express truncated BRCA2 proteins. Functional complementation of FA-D1 fibroblasts with wild-type BRCA2 complementary DNA restores MMC resistance. Our results link the six cloned FA genes with BRCA1 and BRCA2 in a common pathway. Germ-line mutation of genes in this pathway may result in cancer risks similar to those observed in families with BRCA1 or BRCA2 mutations.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Howlett, Niall G -- Taniguchi, Toshiyasu -- Olson, Susan -- Cox, Barbara -- Waisfisz, Quinten -- De Die-Smulders, Christine -- Persky, Nicole -- Grompe, Markus -- Joenje, Hans -- Pals, Gerard -- Ikeda, Hideyuki -- Fox, Edward A -- D'Andrea, Alan D -- P01HL54785/HL/NHLBI NIH HHS/ -- R01DK43889/DK/NIDDK NIH HHS/ -- R01HL52725/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2002 Jul 26;297(5581):606-9. Epub 2002 Jun 13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pediatric Oncology, Children's Hospital, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12065746" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Amino Acid Sequence
;
BRCA2 Protein/chemistry/genetics/*metabolism
;
Cell Line
;
DNA Damage
;
Fanconi Anemia/*genetics
;
Female
;
Fibroblasts
;
Frameshift Mutation
;
Gene Silencing
;
Genes, BRCA1
;
*Genes, BRCA2
;
Genetic Complementation Test
;
Germ-Line Mutation
;
Homozygote
;
Humans
;
Male
;
Mitomycin/pharmacology
;
Molecular Sequence Data
;
*Mutation
;
Pedigree
;
Phenotype
;
Protein Isoforms
;
RNA, Messenger/genetics/metabolism
;
Reverse Transcriptase Polymerase Chain Reaction
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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