Publication Date:
1997-01-31
Description:
Lithographically patterned grids of photoresist, aluminum oxide, or gold on oxidized silicon substrates were used to partition supported lipid bilayers into micrometer-scale arrays of isolated fluid membrane corrals. Fluorescently labeled lipids were observed to diffuse freely within each membrane corral but were confined by the micropatterned barriers. The concentrations of fluorescent probe molecules in individual corrals were altered by selective photobleaching to create arrays of fluid membrane patches with differing compositions. Application of an electric field parallel to the surface induced steady-state concentration gradients of charged membrane components in the corrals. In addition to producing patches of membrane with continuously varying composition, these gradients provide an intrinsically parallel means of acquiring information about molecular properties such as the diffusion coefficient in individual corrals.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Groves, J T -- Ulman, N -- Boxer, S G -- New York, N.Y. -- Science. 1997 Jan 31;275(5300):651-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, Stanford University, Stanford, CA 94305, USA. University, Stanford, CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9005848" target="_blank"〉PubMed〈/a〉
Keywords:
Aluminum Oxide
;
Chemistry, Physical
;
Diffusion
;
Electricity
;
Fluorescent Dyes
;
Light
;
*Lipid Bilayers
;
*Membrane Fluidity
;
Pattern Recognition, Automated
;
Phosphatidylcholines
;
Physicochemical Phenomena
;
Silicon Dioxide
;
Xanthenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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