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  • *Poliovirus  (1)
  • Mice  (1)
  • Mice, Transgenic  (1)
  • American Association for the Advancement of Science (AAAS)  (2)
  • 1
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    Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template (2002)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2002-07-13
    Beschreibung: Full-length poliovirus complementary DNA (cDNA) was synthesized by assembling oligonucleotides of plus and minus strand polarity. The synthetic poliovirus cDNA was transcribed by RNA polymerase into viral RNA, which translated and replicated in a cell-free extract, resulting in the de novo synthesis of infectious poliovirus. Experiments in tissue culture using neutralizing antibodies and CD155 receptor-specific antibodies and neurovirulence tests in CD155 transgenic mice confirmed that the synthetic virus had biochemical and pathogenic characteristics of poliovirus. Our results show that it is possible to synthesize an infectious agent by in vitro chemical-biochemical means solely by following instructions from a written sequence.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cello, Jeronimo -- Paul, Aniko V -- Wimmer, Eckard -- New York, N.Y. -- Science. 2002 Aug 9;297(5583):1016-8. Epub 2002 Jul 11.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-5222, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12114528" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antibodies, Monoclonal/immunology ; Capsid/metabolism ; Cell-Free System ; DNA, Complementary/*chemical synthesis/genetics ; DNA-Directed RNA Polymerases/genetics ; Female ; *Genome, Viral ; HeLa Cells ; Humans ; Male ; *Membrane Proteins ; Mice ; Mice, Transgenic ; Neutralization Tests ; Poliomyelitis/virology ; *Poliovirus/genetics/immunology/pathogenicity/physiology ; Promoter Regions, Genetic ; Protein Biosynthesis ; RNA, Viral/*chemical synthesis/genetics/physiology ; Receptors, Virus/genetics/immunology/metabolism ; Transcription, Genetic ; Viral Plaque Assay ; Viral Proteins ; Virulence ; Virus Replication
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
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    Eradicating polio: A balancing act (2016)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2016-01-23
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Agol, Vadim -- Cello, Jeronimo -- Chumakov, Konstantin -- Ehrenfeld, Ellie -- Wimmer, Eckard -- New York, N.Y. -- Science. 2016 Jan 22;351(6271):348. doi: 10.1126/science.351.6271.348-b.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow, 142782, Russia. A. N. Belozersky Institute of Physical-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, 119899, Russia. ; Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY 11794, USA. ; Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20903, USA. Konstantin.Chumakov@fda.hhs.gov. ; NIAID, NIH, Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26798005" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Disease Eradication ; *Global Health ; Humans ; Poliomyelitis/*prevention & control ; *Poliovirus
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    AKTUELLE ARTIKEL
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