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  • Agrobacterium rhizogenes  (3)
  • Golgi apparatus  (2)
  • Springer  (5)
  • American Association for the Advancement of Science (AAAS)
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  • Springer  (5)
  • American Association for the Advancement of Science (AAAS)
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  • 1
    Electronic Resource
    Electronic Resource
    The fine structure of sublingual gland acinar cells of the Mongolian gerbil, Meriones unguiculatus, processed by rapid freezing followed by freeze-substitution fixation (1987)
    Springer
    Cell & tissue research 250 (1987), S. 305-314 
    Details
    ISSN: 1432-0878
    Keywords: Sublingual gland ; Ultrastructure ; Golgi apparatus ; Rapid-freezing ; Freeze-substitution ; Gerbil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We compare the ultrastructure of the gerbil sublingual gland as seen after cryofixation followed by substitution with osmium tetroxide, with the more familiar appearance of material processed by glutaraldehyde-osmium chemical fixation. After primary cryofixation of fresh salivary glands, the nuclei of the mucous cells are found to be spherical in shape and, rather than being displaced toward the cell base, occupy a nearly central position in the cytoplasm, even in the storage phase of the secretory cycle. The mucous secretory granules are seen as membrane-limited inclusions, only rarely partially fused to each other. In both mucous and serous cells the Golgi cisterns have numerous large fenestrae which are aligned to form cytoplasmic channels which extend across the stack.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219075
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of colchicine on the addition of a sugar to the enamel protein in secretory ameloblasts of cultured germs of rat molar tooth by 3H-galactose radioautography (1990)
    Springer
    Cell & tissue research 260 (1990), S. 521-528 
    Details
    ISSN: 1432-0878
    Keywords: Colchicine ; Golgi apparatus ; Secretory ameloblast ; Glycoprotein synthesis ; Radioautography ; In vitro study ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The influence of colchicine on the addition of 3H-galactose to the enamel protein in secretory amelloblasts of cultured germs of rat molar tooth was investigated by light- and electron-microscopic radioautography. In tooth germs cultured without colchicine, the reaction products of 3H-galactose were observed over Golgi cisternae at early chase times and then localized over the enamel with time. In tooth germs cultured with colchicine, the silver grains were seen over the Golgi cisternae, condensing granules and accumulated secretory granules. Some grains also appeared with time over the pale granular material precipitated in the intercellular space with colchicine treatment. In quantitative analysis with light microscopic radioautography, values of silver grain counts over the unit area (100 μm2) on ameloblasts and enamel of colchicine-treated tooth germs were significantly lower at both 0 min and 30 min chase after 30 min pulse than those of control tooth germs, respectively. This finding indicates that colchicine diminished the incorporation of 3H-galactose into the secretory ameloblast of cultured tooth germs. It is suggested that colchicine decreases the activity of the Golgi apparatus with regared to the addition of sugar to the synthesizing glycoprotein in the secretory ameloblast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297232
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  • 3
    Electronic Resource
    Electronic Resource
    Horizontal gene transfer: regulated expression of a tobacco homologue of the Agrobacterium rhizogenes rolC gene (1995)
    Springer
    Molecular genetics and genomics 249 (1995), S. 265-273 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium rhizogenes ; Horizontal gene transfer ; Evolution ; Ri plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A tobacco homologue (trolC) of the rolC gene of the Agrobacterium rhizogenes Ri-plasmid was cloned and sequenced from Nicotiana tabacum L. cv. Havana 425. The coding region of trolC is similar in sequence (69–87% for DNA and 5489% for the deduced amino acid sequence) to rolC genes of the agropine, mannopine, and mikimopine strains of Ri-plasmids and the N. glauca rolC homologue. Southern analyses showed that trolC is encoded by a small gene family derived from the tomentosiformis ancestor of tobacco. This suggests that trolC resulted from an ancient transfer of DNA between A. rhizogenes and a progenitor of modern tobacco. Transcripts of trolC were detected in three morphologically distinct cultivars of tobacco. trolC mRNA accumulated in young leaves and shoot tips, but not in lower leaves and roots of mature plants. Accumulation of trolC mRNA in cultured leaf tissues was strongly down-regulated by auxin and induced by cytokinin. These results are of particular interest because they suggest that a gene of bacterial origin introduced during evolution can have a function in a modern plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290526
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  • 4
    Electronic Resource
    Electronic Resource
    Evidence for the expression of the rol genes of Nicotiana glauca in genetic tumors of N. glauca x N. langsdorflli (1990)
    Springer
    Molecular genetics and genomics 220 (1990), S. 177-180 
    Details
    ISSN: 1617-4623
    Keywords: Genetic tumor ; Agrobacterium rhizogenes ; rol genes ; Auxin sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thus far, no evidence has been presented that the rol genes (Ng rol) of Nicotiana glauca (Furner et al. 1986) are expressed in this plant. However, we found that the Ng rol genes were transcribed in genetic tumors formed in hybrids of N. glauca x N. langsdorffii. During the culture of such genetic tumors, the level of transcription of Ng rol B increased while that of Ng rol C decreased in parallel with an increase in the endogenous auxin level in the tissues. Moreover, the transcription of these genes was completely suppressed by the application of exogenous auxin. Since the measured endogenous level of auxin was rather low, the formation of tumors in these hybrids can be tentatively explained as a consequence of the expression of rol genes that is responsible for the increased sensitivity to auxin of the hybrids. This novel finding is discussed in relation to the cause of genetic tumors in Nicotiana.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260478
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  • 5
    Electronic Resource
    Electronic Resource
    Sequence of the cellular T-DNA in the untransformed genome of Nicotiana glauca that is homologous to ORFs 13 and 14 of the Ri plasmid and analysis of its expression in genetic tumors of N. glauca x N. langsdorffii (1994)
    Springer
    Molecular genetics and genomics 243 (1994), S. 706-710 
    Details
    ISSN: 1617-4623
    Keywords: Genetic tumors ; Nicotiana glauca T-DNA ; Agrobacterium rhizogenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A region homologous to the TL-DNA of Agrobacterium rhizogenes was previously detected in the genome of untransformed Nicotiana glauca and designated cellular T-DNA (cT-DNA). Subsequently, part of this region was sequenced and two genes, which corresponded to rolB and rolC and were named NgrolB and NgrolC, were found. We have now sequenced a region of the cT-DNA other than the region that includes NgrolB and C and we have found two other open reading frames (ORFs), NgORF13 and NgORF14. These ORFs correspond to ORFs 13 and 14 of the TL-DNA of A. rhizogenes and exhibit a high degree of homology to these ORFs, without having a nonsense codon. We have not found any sequence homologous to rolD (ORF15). The two genes, NgORF13 and 14, as well as the NgrolB and C genes, are expressed in genetic tumors of hybrids between N. glauca and N. langsdorffii but not in leaf tissues of the hybrid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279581
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