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  • Flow cytometry  (3)
  • Springer  (3)
  • American Association for the Advancement of Science (AAAS)
  • Wiley-Blackwell
  • 1
    Electronic Resource
    Electronic Resource
    Monoparametric models of flow cytometric karyotypes with spreadsheet software (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 295-303 
    Details
    ISSN: 1432-2242
    Keywords: Chromosomes ; Flow cytometry ; Theoretical flow karyotypes ; Spreadsheet software
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Theoretical flow karyotypes from both plant and mammalian species have been simply modelled using computer spreadsheet software. The models are based upon published values of relative DNA content or relative lengths of each of the chromosomes. From such data, the histograms of chromosome distribution have been simulated for both linear and logarithmic modules of a flow cytometer, and as a function of the coefficient of variation. Simulated and experimental histograms are compard for Nicotiana plumbaginifolia. This readily accessible exercise facilitates the planning and execution of flow cytometric analysis and sorting of chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266200
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Quantification of nuclear DNA and G-C content in marine macroalgae by flow cytometry of isolated nuclei (1993)
    Springer
    Protoplasma 173 (1993), S. 123-132 
    Details
    ISSN: 1615-6102
    Keywords: Flow cytometry ; Genome size ; G-C composition ; Protoplasts ; Seaweeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The amounts of nuclear DNA in ten species of seaweeds belonging to the Rhodophyceae, Phaeophyceae, and Chlorophyceae were determined by flow cytometric analysis of nuclei isolated from protoplasts. Genome size was determined from the fluorescence of the nuclei stained with ethidium bromide. The size of the nuclear genome ranged from 0.13 pg per cell in the 1 C population ofUlva rigida to 3.40 pg per cell in the 2 C population ofSphacelaria sp. GC% analysis was based on staining with either Hoechst 33342 or mithramycin A, two fluorochromes specific for the bases A-T and G-C, respectively. Two models were used for the estimation of the proportion of guanine plus cytosine in the nuclear genome. The first one was based on the linear relationships mithramycin A fluorescence/G-C content and ethidium bromide fluorescence/total DNA content. The second model, based on the curvilinear relationships Hoechst 33342 fluorescence/A-T content and mithramycin A fluorescence/G-C content, resulted in comparatively more homogenous and consistent data and appears more accurate. Comparison with previous reports from other methods for the physical investigation of nuclear genomes shows that flow cytometry of nuclei isolated from protoplasts is an accurate, convenient and robust technique to assay for genome sizes and base pair composition in marine macroalgae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01379001
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  • 3
    Electronic Resource
    Electronic Resource
    Artificial antisense RNA regulation of YBR1012 (YBR136w ), an essential gene from Saccharomyces cerevisiae which is important for progression through G1/S (1995)
    Springer
    Molecular genetics and genomics 249 (1995), S. 51-57 
    Details
    ISSN: 1617-4623
    Keywords: Antisense RNA ; YBR1012/YBR136w/MEC1/ESR1 ; Saccharomyces cerevisiae ; Flow cytometry ; Cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract YBR1012 (YBR136w) is an essential gene from Saccharomyces cerevisiae identified during the systematic sequencing of part of the right arm of chromosome II. We previously constructed a conditional allele of YBR1012 based on antisense RNA, by inserting a small fragment of this gene downstream from the inducible UASGAL10-CYC1 promoter. Several other antisense RNA constructions have since been made and their activity tested. The response of the system appears to be very delicate, as the presence or absence of 13 nucleotides of polylinker in the 300 nucleotide antisense transcript can dramatically modify its effectiveness. The most effective antisense RNA construction was used in flow cytometry studies to investigate the role of ybr1012p. The results show that during the antisense RNA block some 80% of the cells are arrested with their DNA unreplicated, suggesting that Ybr1012p is needed for progression through G1 or early S phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290235
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