ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Unbekannt

High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh (2018)

Islam, Md Tarikul ; Sarkar, Suprovath Kumar ; Sultana, Nusrat ; [weitere]

BioMed Central

In: BMC Genetics. 2018; 19(1): 1. Published 2018 Jan 02. doi: 10.1186/s12863-017-0594-3.

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2018-01-02

Digitale ISSN: 1471-2156

Thema: Biologie

Publiziert von BioMed Central

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

2

Unbekannt

A molecular assembly phase transition and kinetic proofreading modulate Ras activation by SOS (2019)

Huang, W. Y. C., Alvarez, S., Kondo, Y., Lee, Y. K., Chung, J. K., Lam, H. Y. M., Biswas, K. H., Kuriyan, J., Groves, J. T.

American Association for the Advancement of Science (AAAS)

In: Science

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2019

Beschreibung: 〈p〉The guanine nucleotide exchange factor (GEF) Son of Sevenless (SOS) is a key Ras activator that is autoinhibited in the cytosol and activates upon membrane recruitment. Autoinhibition release involves structural rearrangements of the protein at the membrane and thus introduces a delay between initial recruitment and activation. In this study, we designed a single-molecule assay to resolve the time between initial receptor-mediated membrane recruitment and the initiation of GEF activity of individual SOS molecules on microarrays of Ras-functionalized supported membranes. The rise-and-fall shape of the measured SOS activation time distribution and the long mean time scale to activation (~50 seconds) establish a basis for kinetic proofreading in the receptor-mediated activation of Ras. We further demonstrate that this kinetic proofreading is modulated by the LAT (linker for activation of T cells)–Grb2–SOS phosphotyrosine-driven phase transition at the membrane.〈/p〉

Print ISSN: 0036-8075

Digitale ISSN: 1095-9203

Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik

Publiziert von American Association for the Advancement of Science (AAAS)

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

3

Unbekannt

In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh (2015)

Md. Rahman; Md. Islam; Mohitosh Biswas; A. Khurshid Alam

BioMed Central

In: BMC Research Notes

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2015-10-31

Beschreibung: Background: In humans, many diseases are associated with the accumulation of free radicals. Antioxidants can scavenge free radicals and minimize their impact. Therefore, the search for naturally occurring antioxidants of plant origin is imperative. Here, we aimed to investigate the antioxidant and free radical scavenging properties of methanolic extracts from Tabebuia pallida (T. pallida) stem bark (TPSB), root bark (TPRB), leaves (TPL), and flowers (TPF). Methods: The antioxidant and free radical scavenging activity were determined by several standard methods using spectrophotomer. Total phenolic and flavonoid contents were estimated using Folin-Ciocalteu reagent and aluminum chloride colorimetric assay methods, respectively. Results: Among the extracts, TPL showed the highest total antioxidant capacity followed by TPRB, TPF, and TPSB. Based on DPPH and hydroxyl radical scavenging activity, TPL showed strong scavenging activity (91.05 ± 1.10 and 62.00 ± 0.57) with IC 50 of 9.20 ± 0.28 and 46.00 ± 2.84 μg/mL, respectively when compared with standard BHT (IC 50 of 7.00 ± 0.25 μg/mL) and CA (75.00 ± 0.14 μg/mL). These results suggest that TPL had the highest radical scavenging activity among the extractives that closely resembled the standard’s. In lipid peroxidation inhibition assay, TPL exhibited the most potent inhibitory activity (83.18 ± 2.12 %) with IC 50 of 12.00 ± 2.12 μg/mL, which closely resembled standard CA (IC 50 of 10.50 ± 0.28 μg/mL). Also, the reducing capacity on ferrous ion was in the following order: TPL 〉 TPRB 〉 TF 〉 TPSB. The phenolic and flavonoid contents of TPL were higher than other extractives. A positive correlation (p value

Digitale ISSN: 1756-0500

Thema: Biologie , Medizin

Publiziert von BioMed Central

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

4

Unbekannt

A metagenomic strategy for harnessing the chemical repertoire of the human microbiome (2019)

Sugimoto, Y., Camacho, F. R., Wang, S., Chankhamjon, P., Odabas, A., Biswas, A., Jeffrey, P. D., Donia, M. S.

American Association for the Advancement of Science (AAAS)

In: Science

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2019

Beschreibung: 〈p〉Extensive progress has been made in determining the effects of the microbiome on human physiology and disease, but the underlying molecules and mechanisms governing these effects remain largely unexplored. Here, we combine a new computational algorithm with synthetic biology to access biologically active small molecules encoded directly in human microbiome–derived metagenomic sequencing data. We discover that members of a clinically used class of molecules are widely encoded in the human microbiome and that they exert potent antibacterial activities against neighboring microbes, implying a possible role in niche competition and host defense. Our approach paves the way toward a systematic unveiling of the chemical repertoire encoded by the human microbiome and provides a generalizable platform for discovering molecular mediators of microbiome-host and microbiome-microbiome interactions.〈/p〉

Print ISSN: 0036-8075

Digitale ISSN: 1095-9203

Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik

Publiziert von American Association for the Advancement of Science (AAAS)

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

5

Unbekannt

A Modular Gain-of-Function Approach to Generate Cortical Interneuron Subtypes from ES Cells (2013)

Au, Edmund ; Ahmed, Tanzeel ; Karayannis, Theofanis ; [weitere]

Cell Press

In: Neuron. 2013; 80(5): 1145-1158. Published 2013 Dec 01. doi: 10.1016/j.neuron.2013.09.022.

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2013-12-01

Print ISSN: 0896-6273

Digitale ISSN: 1097-4199

Thema: Biologie , Medizin

Publiziert von Cell Press

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

6

Unbekannt

A novel GPR40 agonist, CNX-011-67, suppresses glucagon secretion in pancreatic islets under chronic glucolipotoxic conditions in vitro (2014)

Mahesh Kumar Verma; Sanghamitra Biswas; Bhawna Chandravanshi; Korrapati Neelima; Anup Oommen; Madanahalli Jagannath; Baggavalli Somesh

BioMed Central

In: BMC Research Notes

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2014-09-04

Beschreibung: Background: Elevated glucose concentrations lead to increased insulin secretion and suppression of glucagon secretion. In fact, insulin is a physiological inhibitor of glucagon secretion. Type 2 diabetes mellitus (T2DM) patients have defects in insulin secretion. In addition to this, lack of suppression of glucagon secretion under elevated glucose concentrations is also observed in T2DM patients. We have earlier shown that GPR40 activation by CNX-011-67 stimulates glucose stimulated insulin secretion (GSIS). Here we extended our studies to examine the impact of GPR40 activation by CNX-011-67 on glucagon secretion from intact islets under both normal and glucolipotoxic conditions.FindingsGlucagon secretion from intact rat islets was suppressed under elevated glucose concentration. Activation of GPR40 by CNX-011-67 further suppressed glucagon secretion. Culturing islets under chronic glucolipotoxic (GL) conditions, we have observed increased high glucose mediated glucagon secretion and content which were reduced with GPR40 activation by CNX-011-67. Interestingly, expression of pre-proglucagon gene (GCG) remained unchanged under glucolipotoxicity in the presence or absence of GPR40 activation. Conclusion: Activation of GPR40 by CNX-011-67 can reduce glucagon secretion from pancreatic islets.

Digitale ISSN: 1756-0500

Thema: Biologie , Medizin

Publiziert von BioMed Central

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

7

Unbekannt

Molecular cloning and in-silico characterization of high temperature stress responsive pAPX gene isolated from heat tolerant Indian wheat cv. Raj 3765 (2014)

Jasdeep Padaria; Harinder Vishwakarma; Koushik Biswas; Rahul Jasrotia; Gyanendra Singh

BioMed Central

In: BMC Research Notes

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2014-10-11

Beschreibung: Background: Heat stress leads to accelerated production of reactive oxygen species (ROS) which causes a huge amount of oxidative damage to the cellular components of plants. A large number of heat stress related genes as HSPs, catalases, peroxidases are overexpressed at the time of stress. A potent stress responsive gene peroxisomal ascorbate peroxidase (TapAPX) obtained from heat stress (42[degree sign]C) responsive subtractive cDNA library from a thermo tolerant wheat cv. Raj3765 at anthesis stage was cloned, characterized and its role was validated under heat stress by proteomics and in-silico studies.. In the present study we report the characterization at molecular and in-silico level of peroxisomal TapAPX gene isolated from heat tolerant wheat cultivar of India. Results: qPCR studies of TapAPX gene displayed up to 203 fold level of expression at 42[degree sign]C heat stress exposure. A full length cDNA of 876 bp obtained by RACE deduced a protein of 292 amino acid residues which gives a complete 3D structure of pAPX by homology modeling. TapAPX cDNA was cloned in expression vector pET28 (a+) and the recombinant protein over-expressed in E. coli BL21 showed highest homology with APX protein as deduced by peptide mass fingerprinting. Conclusions: TapAPX gene from wheat cv Raj3765 has a distinct role in conferring thermo tolerance to the plants and thus can be used in crop improvement programmes for development of crops tolerant to high temperature.

Digitale ISSN: 1756-0500

Thema: Biologie , Medizin

Publiziert von BioMed Central

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

8

Unbekannt

Procalcitonin as a prognostic marker for sepsis: a prospective observational study (2014)

Saransh Jain; Sanjeev Sinha; Surendra Sharma; J Samantaray; Praveen Aggrawal; Naval Vikram; Ashutosh Biswas; Seema Sood; Manish Goel; Madhuchhanda Das; Sreenivas Vishnubhatla; Nawaid Khan

BioMed Central

In: BMC Research Notes

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2014-07-18

Beschreibung: Background: Procalcitonin is useful for the diagnosis of sepsis but its prognostic value regarding mortality is unclear. This prospective observational study was designed to study the prognostic value of procalcitonin in prediction of 28 day mortality in patients of sepsis. Fifty-four consecutive patients of sepsis, severe sepsis and septic shock defined using the 2001 Consensus Conference SCCM/ESICM/ACCP/ATS/SIS criteria from medical Intensive Care Unit (ICU) of a tertiary care center in New Delhi, India were enrolled from July 2011 to June 2013. Procalcitonin (PCT), C-reactive protein (CRP) measurements were recorded on day 1, day 7 and day 28 of follow up. Results: Procalcitonin value was a better predictor of all-cause short-term mortality than C-reactive protein. Those patients with Procalcitonin levels

Digitale ISSN: 1756-0500

Thema: Biologie , Medizin

Publiziert von BioMed Central

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

9

Unbekannt

Direct observation of percolation in a manganite thin film (2002)

L. Zhang ; C. Israel ; A. Biswas ; [weitere]

American Association for the Advancement of Science (AAAS)

In: Science. 298(5594): 805-7. doi: 10.1126/science.1077346.

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2002-09-21

Beschreibung: Upon cooling, the isolated ferromagnetic domains in thin films of La0.33Pr0.34Ca0.33MnO3 start to grow and merge at the metal-insulator transition temperature TP1, leading to a steep drop in resistivity, and continue to grow far below TP1. In contrast, upon warming, the ferromagnetic domain size remains unchanged until near the transition temperature. The jump in the resistivity results from the decrease in the average magnetization. The ferromagnetic domains almost disappear at a temperature TP2 higher than TP1, showing a local magnetic hysteresis in agreement with the resistivity hysteresis. Even well above TP2, some ferromagnetic domains with higher transition temperatures are observed, indicating magnetic inhomogeneity. These results may shed more light on the origin of the magnetoresistance in these materials.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, Liuwan -- Israel, Casey -- Biswas, Amlan -- Greene, R L -- de Lozanne, Alex -- New York, N.Y. -- Science. 2002 Oct 25;298(5594):805-7. Epub 2002 Sep 19.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics, University of Texas, Austin, TX 78712, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12242450" target="_blank"〉PubMed〈/a〉

Print ISSN: 0036-8075

Digitale ISSN: 1095-9203

Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik

Publiziert von American Association for the Advancement of Science (AAAS)

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext

10

Unbekannt

Polymorphisms at the F12 and KLKB1 loci have significant trait association with activation of the renin-angiotensin system (2016)

Nilima Biswas, Adam X. Maihofer, Saiful Anam Mir, Fangwen Rao, Kuixing Zhang, Srikrishna Khandrika, Manjula Mahata, Ryan S. Friese, C. Makena Hightower, Sushil K. Mahata, Dewleen G. Baker, Caroline M. Nievergelt, Sucheta M. Vaingankar and Daniel T. O’Connor

BioMed Central

In: BMC Medical Genetics

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2016-03-12

Beschreibung: Plasma coagulation Factor XIIa (Hageman factor; encoded by F12) and kallikrein (KAL or Fletcher factor; encoded by KLKB1) are proteases of the kallikerin-kinin system involved in converting the inactive circulati...

Digitale ISSN: 1471-2350

Thema: Biologie , Medizin

Publiziert von BioMed Central

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

AKTUELLE ARTIKEL

S·F·X

Volltext