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  • 1
    Electronic Resource
    Electronic Resource
    An almost ideal demand system for alcoholic beverages in British Columbia (1992)
    Springer
    Empirical economics 17 (1992), S. 401-418 
    Details
    ISSN: 1435-8921
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract An almost ideal demand system for alcoholic beverages in British Columbia is estimated based on five beverage categories. Estimates of the model unrestricted and restricted to satisfy homogeneity and symmetry are presented. The restrictions are tested: as is common in applied demand analysis a number of rejections are encountered, although within-equation tests tend to support homogeneity. The rejections which are encountered are not mitigated by the inclusion of dynamic elements. The Slutsky matrix is used to examine the concavity of the expenditure function, which is found to be mildly violated. Marshallian and Hicksian own-, cross-price, and income elasticities are calculated and are found to be largely consistent with previous findings, although some noteworthy results are obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01206301
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  • 2
    Electronic Resource
    Electronic Resource
    [4-proline, 8-isoleucine]-oxytocin and [4-leucine, 8-isoleucine]-oxytocin, possible intermediates in the evolutionary series of neurohypophysial hormones: Synthesis and some pharmacological properties (1969)
    Springer
    Cellular and molecular life sciences 25 (1969), S. 680-682 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung [4-Prolin, 8-Isoleucin]-Oxytocin und [4-Leucin, 8-Isoleucin]-Oxytocin wurden als mögliche Glieder in der entwicklungsgeschichtlichen Reihe der Neurohypophysenhormone synthetisiert und pharmakologisch geprüft.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01897555
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  • 3
    Electronic Resource
    Electronic Resource
    Thermal analysis of chromium(III) and chromium(VI) systems with silica and sodium silicate (1976)
    Springer
    Journal of thermal analysis and calorimetry 9 (1976), S. 79-82 
    Details
    ISSN: 1572-8943
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Résumé Le silicate de sodium ou un mélange de silice et de carbonate de sodium réagissent, en présence d'oxygène, avec l'oxyde de chrome trivalent pour former le chromate de sodium, approximativement entre 300 et 900°. Au-dessus de 900°, la réaction s'inverse et l'oxyde de chrome(III) est régénéré.
    Abstract: Zusammenfassung Natriumsilikat oder eine Mischung von Siliziumdioxid mit Natriumkarbonat ergeben bei der Reaktion mit Chrom(III)oxid in Gegenwart von Luft im Temperaturbereich zwischen 300° und 900° Natriumchromat. Über 900° wird die Reaktion umgekehrt und Chrom(III)oxid zurückgebildet.
    Notes: Abstract Sodium silicate, or a mixture of silica and sodium carbonate, reacts with chromium(III) oxide in the presence of oxygen to give sodium chromate, within the approximate temperature range 300–900°. Above 900° the reaction is reversed and chromium(III) oxide regenerated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01909268
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  • 4
    Electronic Resource
    Electronic Resource
    Regulation of rat cardiac nuclei-associated Mg2+-NTPase by phosphorylation (1991)
    Springer
    Molecular and cellular biochemistry 102 (1991), S. 165-172 
    Details
    ISSN: 1573-4919
    Keywords: rat heart ; nuclei ; phosphorylation ; ATPase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract A nucleoside triphosphatase (NTPase) activity appeared to be associated with a highly purified nuclear preparation from rat cardiac ventricles. Different nucleoside triphosphates (UTP 〉 GTP 〉 ITP 〉 CTP) supported this enzymic activity, which was stimulated by Mg` but not by Call. The nuclear NTPase activity could be down regulated by endogenous phosphorylation of a 55,000 Mr protein. Maximal phosphorylation of the 55,000 Mr protein occurred in the presence of Mg2+-ATP. Addition of cAMP, cGMP, Ca2+, Ca2+/phospholipid, Ca2+/calmodulin, and catalytic subunit of cAMP-dependent protein kinase was not associated with any further phosphorylation of the 55,000 Mr protein. However, in the presence of Ca2+/calmodulin or the catalytic subunit of the cAMP-dependent protein kinase additional proteins became phosphorylated, but these had no effect on the Mg2+-NTPase activity. These results indicate that a protein with Mr 55,000 may be involved in the regulation the Mg2+-NTPase activity associated with rat cardiac nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234574
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  • 5
    Electronic Resource
    Electronic Resource
    The morphology of the toad urinary bladder: A stereoscopic and transmission electron microscopical study (1970)
    Springer
    Cell & tissue research 109 (1970), S. 297-305 
    Details
    ISSN: 1432-0878
    Keywords: Bladder ; Bufo marinus ; Transport ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The appearance of the mucosal cell layer of the isolated urinary bladder of the toadBufo marinus, has been examined using stereoscopic and conventional transmission electron microscopy. Three cell types can be identified in surface view, these are granular cells, mitochondriarich cells and goblet cells. Cell boundaries between granular cells are clearly defined by membranous folds along their margins. Although no changes are seen in the stereoscopic electron micrographs when the granular cells are made permeable to water by vasopressin, the changes observed on transmission electron micrographs include swelling of cell bodies and nuclei, filling of intercellular channels with water, and the appearance beneath the mucosal cell membrane surface of electron dense granules. Differences between the appearance of the bladder mucosal cells by the two methods of electron microscopical examination are due largely to water loss when the tissue is freeze dried prior to stereoscopic examination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02226903
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  • 6
    Electronic Resource
    Electronic Resource
    An ultrastructural study of mitosis and cytokinesis in normal ‘resting’ human breast (1988)
    Springer
    Cell & tissue research 252 (1988), S. 581-587 
    Details
    ISSN: 1432-0878
    Keywords: Normal resting breast ; Ultrastructure ; Mitosis ; Cytokinesis ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The parenchyma of the normal “resting” human breast was examined by electron microscopy to characterize the cells undergoing mitosis and the mechanism by which the normal tissue architecture is maintained during this process. In this study of 112 mitotic cells, it was found that the mitotic cells were luminally positioned, polarised epithelial cells with no evidence of myoepithelial cell division. Ultrastructurally, the nuclear and cytoplasmic changes were consistent with previous reports of mitosis in other tissues. However, unlike all previous reports, two specific orientations of the nuclear spindle and thus the planes of cytokinesis were observed. In a few cases the spindle formed parallel to the lumen and division resulted in two luminally positioned daughter cells. However, in the majority of mitotic cells the spindle was approximately at right angles to the lumen and this orientation resulted in a luminally and a basally positioned daughter cell. It is proposed that the abnormally positioned basal daughter cell could develop into a myoepithelial cell or undergo deletion (apoptosis). Thus the two orientations of mitosis may explain the mechanism by which the epithelial and myoepithelial cell populations were maintained by a single progenitor cell without disrupting the integrity of the tissue architecture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216645
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  • 7
    Electronic Resource
    Electronic Resource
    Petroanalysis '81-advances in analytical chemistry in the petroleum industry 1975–1982 (1983)
    Springer
    Chromatographia 17 (1983), S. 507-507 
    Details
    ISSN: 1612-1112
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02263275
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  • 8
    Electronic Resource
    Electronic Resource
    Combined immunocytochemistry and non-isotopic in situ hybridization for the ultrastructural investigation of human parvovirus B19 infection (1995)
    Springer
    Journal of molecular histology 27 (1995), S. 46-53 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Parvovirus B19 is a single-stranded DNA virus with a specific tropism for human erythroid precursor cells. The virus codes for two overlapping structural (capsid) proteins and one non-structural protein which is thought to perform essential functions in viral replication, transcription and packaging. The ultrastructural localization of these proteins was achieved in cultured haemopoietic cells derived from fetal liver which had been infected in vitro and subsequently embedded in LR White acrylic resin. Postembedding immunogold detection of B19 structural and non-structural proteins was combined with localization of viral nucleic acid by in situ hybridization using a digoxigenin-labelled probe and different sized gold labels. The majority of the B19 capsid protein and DNA present in cells harvested 48 hours post-infection co-localized within the centri-nuclear region of erythroid cells demonstrating characteristic chromatin margination. Relatively little DNA hybridization signal was present over paracrystalline inclusions strongly labelled with anti-capsid protein monoclonal antibody R92F6. Viral DNA and capsid protein were co-localized in apparent egress from the nucleus through nuclear pores. B19 non-structural protein was detected in association with both nuclear and cytoplasmic arrays of capsids, supporting the view that this protein plays an important role in viral packaging and remains associated with the complete viral particle until its release from the cell. Co-localization of viral nucleic acid and proteins at the ultrastructural level is a flexible, rapid and highly specific tool for examination of viral life-cycles within cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00164171
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  • 9
    Electronic Resource
    Electronic Resource
    Intracellular localization of parvovirus B19 nucleic acid at the ultrastructural level by in situ hybridization with digoxigenin-labelled probes (1993)
    Springer
    Journal of molecular histology 25 (1993), S. 421-429 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Conditions suitable for immunogold detection of digoxigenin-labelled DNA probes hybridized to parvovirus B19-infected erythroid cells embedded in Lowicryl K4M and LR White acrylic resins were established at the electron microscope level. The protocol was initially optimized using a positive control probe for whole human DNA which produced signal over the heterochromatin of all nucleated cells. In cultures harvested 2 days postinfection, B19 nucleic acid was detected mainly within the centrinuclear region of erythroid cells exhibiting characteristic margination of the chromatin. The B19 hybridization signal was largely unaffected by denaturation and was resistant to RNase digestion but sensitive to DNase digestion, indicating that it was mainly single-stranded B19 DNA. Relatively few gold particles were found over crystalline arrays of viral capsids, consistent with the observation that they are composed of mainly ‘empty’ capsids. B19 nucleic acid was detected in apparent transit from nucleus to cytoplasm through pores in the nuclear membrane. While the sensitivity of this system is limited by the fact that hybridization occurs only at the surface of the section, it is a rapid and specific means of localizing viral nucleic acids with a high degree of resolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00157806
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  • 10
    Electronic Resource
    Electronic Resource
    Chromosomal localization of genes by scanning electron microscopy usingin situ hybridization with biotinylated probes: Y chromosome repetitive sequences (1986)
    Springer
    Journal of molecular histology 18 (1986), S. 266-270 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The feasibility of using scanning electron microscopy (SEM) to identify the position of specific DNA sequences was examined using a Y chromosome ‘specific’ probe (pHY2.1). Tests were carried out on chromosome spreads hybridizedin situ with biotinylated pHY2.1. Chromosomal sites of hybridization of the probe were localized by an indirect immunohistochemical procedure which resulted in a gold product which could be amplified by silver precipitation. In the SEM, the specific location of the probe was easily identified due to the enhanced signal produced by the gold—silver complex. The probe was localized both on the long arm of the Y chromosome and within interphase nuclei. It was found that SEM was more sensitive than light microscopy since the probe could be identified without silver amplification. With refinements to the technique, SEM could provide a useful method for high resolution localizing of unique DNA sequences (i.e. single copy genes).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01676236
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