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  • Actin  (1)
  • Springer  (1)
  • American Association for the Advancement of Science
  • Institute of Physics
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  • Springer  (1)
  • American Association for the Advancement of Science
  • Institute of Physics
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    Electronic Resource
    Electronic Resource
    Ultrastructural immunolocalization of actin in a fungus (1991)
    Springer
    Protoplasma 163 (1991), S. 199-202 
    Details
    ISSN: 1615-6102
    Keywords: Actin ; Freeze substitution ; Fungi ; Hyphal tip ; Immunocytochemistry ; Magnaporthe grisea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have successfully localized fungal actin for the first time using immuno-electron microscopy and hyphal tips of the rice blast pathogenMagnaporthe grisea. Following ultrarapid freezing, samples were processed in a novel substitution fluid of 10% acrolein in anhydrous ethanol and embedded in LR White resin. A monoclonal anti-actin antibody, previously shown to recognizeM. grisea actin, bound specifically to filasomes concentrated in the peripheral cytoplasm of subapical regions, and to the core-region of the Spitzenkörper.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01323344
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