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  • American Association for the Advancement of Science  (2)
  • Genetics Society of America (GSA)  (1)
  • Sage  (1)
  • 1
    Publication Date: 2016-04-08
    Description: The eastern North American monarch butterfly, Danaus plexippus , is an emerging model system to study the neural, molecular, and genetic basis of animal long-distance migration and animal clockwork mechanisms. While genomic studies have provided new insight into migration-associated and circadian clock genes, the general lack of simple and versatile reverse-genetic methods has limited in vivo functional analysis of candidate genes in this species. Here, we report the establishment of highly efficient and heritable gene mutagenesis methods in the monarch butterfly using transcriptional activator-like effector nucleases (TALENs) and CRISPR-associated RNA-guided nuclease Cas9 (CRISPR/Cas9). Using two clock gene loci, cryptochrome 2 and clock ( clk ), as candidates, we show that both TALENs and CRISPR/Cas9 generate high-frequency nonhomologous end-joining (NHEJ)-mediated mutations at targeted sites (up to 100%), and that injecting fewer than 100 eggs is sufficient to recover mutant progeny and generate monarch knockout lines in about 3 months. Our study also genetically defines monarch CLK as an essential component of the transcriptional activation complex of the circadian clock. The methods presented should not only greatly accelerate functional analyses of many aspects of monarch biology, but are also anticipated to facilitate the development of these tools in other nontraditional insect species as well as the development of homology-directed knock-ins.
    Electronic ISSN: 2160-1836
    Topics: Biology
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  • 2
    Publication Date: 2013-04-19
    Description: Between 1857 and 1860 the British North American Expedition, led by Captain John Palliser, explored and surveyed the Canadian Prairies primarily to establish its suitability for agriculture and settlement. Historical and paleoclimate records indicate the Expedition coincided with below normal precipitation, leading to the perception of an arid or semi-arid region that would ‘forever be comparatively useless’ for agriculture. Today, this part of the Canadian Prairies is known as the Palliser Triangle, and is Canada’s productive dryland agricultural region. Here we present historic, geomorphologic, and chronometric evidence to reconstruct the landscape encountered by the Expedition. We contend that Palliser’s perception of the region was strongly influenced by his experience travelling through active sand dunes in the Middle Sand Hills of southeastern Alberta. At present, the dunes are entirely stabilized by vegetation, in contrast to Palliser’s report of ‘miles of burning sand’. Archival aerial photographs and optical ages of near-surface samples are used to reconstruct the landscape encountered by the Expedition in the Middle Sand Hills. Optical ages of presently stabilized sand dunes date primarily to between ad 1850 and 1934, peaking in c . ad 1925, and are indicative of a dune field undergoing reduction in activity, prior to the onset of 20th century droughts. Ages of interdune sand sheets further attest to regional dune activity occurring at least since ad 1750, concurrent with activity in other southern Canadian Prairie dune fields. Collectively, this evidence supports observations by Palliser of severe travelling due to bare sand conditions in 1857–1859. These conditions and Palliser’s inference of their extent influenced his perception of a ‘central desert’, thus delaying construction of the Canadian Pacific Railway along a southern route and postponing the westward colonization of Canada.
    Print ISSN: 0959-6836
    Electronic ISSN: 1477-0911
    Topics: Geography , Geosciences
    Published by Sage
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  • 3
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    American Association for the Advancement of Science
    Publication Date: 1982-10-01
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Publication Date: 1984-05-11
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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