ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The RNA-dependent RNA polymerase (P2) from bacteriophage Φ6 has been cloned and the protein overexpressed in Escherichia coli to produce an active enzyme. A fully substituted selenomethionyl version of the protein has also been produced. Crystals of both proteins have been grown; most belong to the monoclinic space group P21, with unit-cell parameters a = 105.9, b = 94.0, c = 140.9 Å, β = 101.4°, but some are trigonal (space group P31 or P32), with unit-cell parameters a = b = 110.1, c = 159.4 Å, γ = 120°. Both crystal forms occur in the same crystallization drop and are morphologically indistinguishable. Native data sets have been collected from both types of crystals to better than 3 Å resolution.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900010702
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