ISSN:
1432-2242
Keywords:
Beta vulgaris
;
Cytoplasmic male sterility
;
mtDNA
;
Restriction fragment patterns
;
Filter hybridization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Mitochondrial (mt) DNA from eight cytoplasmic male-sterile (cms) lines of sugar beet from different breeding stations was investigated by restriction fragment analysis and Southern hybridization. All cms lines showed similar but not identical restriction and hybridization signal patterns, readily distinguishable from those of fertile (N) cytoplasm. Digestion of the mtDNA with BamHI, EcoRI, SalI, and XhoI revealed distinct differences between the sterile lines, and six subtypes of the S cytoplasm could be distinguished. Differences between the sterile lines were confirmed by hybridization with a $$\overline{\overline {COX}} II$$ gene probe revealing minor, line-specific hybridization signals. The data presented provide evidence for the existence of considerable variation within the only commercially used source of cms in the sugar beet, the Owen's type of cytoplasm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00231271
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