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  • Oryza sativa  (1)
  • Pluronic F-68  (1)
  • Springer  (2)
  • Institute of Physics
  • Wiley
  • 1955-1959  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Genetic engineering of Indica rice in support of sustained production of affordable and high quality food in developing countries (1955)
    Springer
    Euphytica 85 (1955), S. 441-449 
    Details
    ISSN: 1573-5060
    Keywords: Oryza sativa ; Indica-type rice ; genetic engineering ; vitamin A endosperm ; insect resistance ; virus resistance ; fungus resistance ; essential amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Indica-type rice provides the staple food for two billion people in Third World countries. Several problems involved in the stable and sustained production of high quality food cannot be solved by traditional breeding. Methods have been established for gene transfer to Indica rice breeding lines to study possible contributions from genetic engineering. Experiments are in progress on the development of transgenic resistance towards Yellow Stem Borer, resistance towards Rice Tungro Virus, accumulation of provitamin A in the endosperm, increase of essential amino acids in the endosperm such as lysine, cysteine and methionine and resistance towards fungal pests such as Rice Blast and Sheath Blight. Transgenic clones from Indica rice breeding lines have been recovered from several of the approaches mentioned, some of which have been regenerated to plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023978
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  • 2
    Electronic Resource
    Electronic Resource
    Improved plant heterokaryon formation by surfactant-supplementation of polyethylene glycol fusogen solution (1955)
    Springer
    Euphytica 85 (1955), S. 269-273 
    Details
    ISSN: 1573-5060
    Keywords: heterokaryon formation ; Petunia ; Pluronic F-68 ; polyethylene glycol ; protoplast fusion ; surfactant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary PEG fusion solution for leaf protoplasts of Petunia parodii and cell suspension protoplasts of albino P. hybrida cv. Comanche was supplemented with 0.01–1.0% (w/v) Pluronic F-68. This stimulated protoplast fusion overall, including parental homokaryon formation, with increased means of 23% and 83% respectively, over appropriate controls using 1.0% (w/v) surfactant added to the standard PEG solution. Interestingly, the percentage heterokaryon formation increased near 2-fold (P〈0.001) for fusogen solutions supplemented with 0.01% (w/v) Pluronic. Protoplasts regenerated to colonies in KM8P/KM8 liquid medium, indicating no adverse effects of Pluronic F-68 on viability, both in the short and longer terms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023955
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    Paper (German National Licenses)
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