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  • N-nitroso-N-ethylurea  (1)
  • Oryza sativa  (1)
  • Springer  (2)
  • Institute of Physics
  • International Union of Crystallography
  • Wiley
  • 1955-1959  (2)
Collection
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  • Springer  (2)
  • Institute of Physics
  • International Union of Crystallography
  • Wiley
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Selection of hydroxyproline-resistant proline-accumulating mutants of cauliflower (brassica oleracea var. botrytis) (1955)
    Springer
    Euphytica 85 (1955), S. 329-334 
    Details
    ISSN: 1573-5060
    Keywords: curd culture ; frost tolerance ; N-nitroso-N-ethylurea ; proline accumulation ; mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A procedure is described by which hydroxyproline-resistant lines could be selected from regenerating curd tissue of cauliflower. Mutagenesis was by N-nitroso-N-ethylurea, supplied as a drop of 0.3 mM solution on each 3 mm diameter curd piece. The mutagen generated numerous morphological and pigment mutations without significantly affecting shoot regeneration from explants. Thirty one resistant shoots were recovered from more than six thousand explants mutagenised on regeneration medium supplemented with 3 mM hydroxyproline, while none was obtained from a similar number of non-mutagenised controls. Out of twenty-three resistant shoots which survived subculture, only one showed consistently elevated levels of endogenous proline. During early shoot culture passages, proline levels were 3.6–4.7 times higher than controls, but this was reduced to 1.6 times after 10–12 culture passages in the absence of hydroxyproline. Possible reasons for this decline are discussed. Leaf strip assays suggest resistant shoots may be chimeras and current efforts are directed towards regenerating solid mutants from resistant sectors. These will then be evaluated for any alteration in frost tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023963
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic engineering of Indica rice in support of sustained production of affordable and high quality food in developing countries (1955)
    Springer
    Euphytica 85 (1955), S. 441-449 
    Details
    ISSN: 1573-5060
    Keywords: Oryza sativa ; Indica-type rice ; genetic engineering ; vitamin A endosperm ; insect resistance ; virus resistance ; fungus resistance ; essential amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Indica-type rice provides the staple food for two billion people in Third World countries. Several problems involved in the stable and sustained production of high quality food cannot be solved by traditional breeding. Methods have been established for gene transfer to Indica rice breeding lines to study possible contributions from genetic engineering. Experiments are in progress on the development of transgenic resistance towards Yellow Stem Borer, resistance towards Rice Tungro Virus, accumulation of provitamin A in the endosperm, increase of essential amino acids in the endosperm such as lysine, cysteine and methionine and resistance towards fungal pests such as Rice Blast and Sheath Blight. Transgenic clones from Indica rice breeding lines have been recovered from several of the approaches mentioned, some of which have been regenerated to plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023978
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    Paper (German National Licenses)
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