ISSN:
1573-5060
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Results of experiments on collection, viability testing, storage and dispatch of coconut pollen are given, and their relevance to the breeding of coconuts is briefly discussed. Pollen was obtained in large quantities following oven-drying of male flowers at 40° C. Viability decreased as drying time increased but viable grains were obtained in greater numbers after two days than after drying for a single day. Germination of pollen in vitro was better at 30° C and 35° C than at other temperatures tried; media with high gelatine concentrations (30%) seemed superior to those with less gelatine. For normal breeding purposes storage of pollen for two or three months is adequate. At low temperature, reliable storage in sealed ampoules for at least this period was obtained after further drying pollen over silica gel. Some samples retained good viability for considerably longer. Most samples stored over silica gel were still viable after 5 months, though viability was low. Over damp CaCl2 considerable reduction in viability occurred during the first month but there was little further reduction at least up to 7 months after collection, and all samples were still viable after 18 months. Freeze-dried pollen in ampoules under vacuum had up to 40% viability after storage for one year. At room temperature, viability of pollen kept at controlled humidities over sulphuric acid solutions, though retained for longer periods than in an uncontrolled atmosphere, was only reliably maintained for about three weeks; some samples remained viable for longer periods. Freeze-drying was found to greatly increase the longevity of pollen kept at room temperature; freeze-dried pollen used in a trial shipment to New Guinea retained viability for four months and was successfully used in a number of controlled pollinations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00022353
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