ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Native Taka-amylase A (α-amylase produced by Asp. oryzae) was coupled with 1-dimethylaminonaphthalene-5-sulfonyl chloride. When disulfide bonds of the modified enxyme were split by reduction or by reduction and subsequent carboxymethylation, its fluorescent properties changed markedly. It was suggested that the hydrophobic dye group is incorporated into a hydrophobic region as the consequence of the flexibility gained by the polypeptide chain by the cleavage of disulfide bonds.
Additional Material:
12 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1967.360050704
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