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RNA Synthesis and Cell Division in Heat-Synchronized Populations of Tetrahymena pyriformis (1965)

MONER, J. G.

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 12 (1965), S. 0

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ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: SYNOPSIS. The incorporation of H3-uridine during the pretransition period of heat-synchronized cells of Tetrahymena pyriformis occurs in two peaks, one at 20 and the other at approximately 40 min following the end of the last synchronizing heat treatment (EH). Although Actinomycin D tends to produce substantial inhibition of both peaks it has a significant effect on cell division only if applied before or during the first peak of incorporation. It appears that the production of new RNA is required for the first synchronous division and that it is synchronized with the division process. Actinomycin D seems to increase, slightly, the degree of incorporation of H3-uridine into RNA when applied at 0 and 10 min past EH. This effect may be correlated with the positive excess delays of division which result when cells are exposed to the inhibitor at these times. A similar effect of Actinomycin on incorporation at 30 or 40 minutes past EH, however, does not appear to be associated with any strong effects of the inhibitor on division.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1965.tb03249.x

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Electronic Resource

RNA synthesis and cell division in cold-synchronized cells of Tetrahymena pyriformis (1966)

Moner, J. G. ; Berger, R. O.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 67 (1966), S. 217-223

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Cells of Tetrahymena pyriformis have been cold-synchronized using a repetitive cycle of six, two-hour cold shocks (9.5°C) alternating with decreasing periods (60-30 minutes) at 28°C. This system gives a maximum division index of 70-80% occurring at 90 minutes from the end of the last synchronizing cold-treatment (EC). Examination of the division sensitivity of these cells to actinomycin D applied continuously at ten-minute intervals from EC reveals that division is essentially blocked until approximately 40 minutes past EC, after which a rapid decrease is sensitivity to the inhibitor occurs. Coinciding with this period of high sensitivity is the occurrence of a peak of C14 uridine incorporation at 40 minutes past EC. Inhibition of this peak is correlated with an inhibition of division, whereas strong inhibition of RNA synthesis beyond 60 minutes past EC has little effect on division activity. The similarity of these findings with those of the heat-synchronized system is discussed with the suggestion that both heat- and cold-synchronizing treatments result in the synchronous resynthesis of a division-associated fraction of RNA.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jcp.1040670203

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