ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Procedures have been devised for the isolation of the surface membranes of mouse fibroblasts (L cell) and a variety of other cells. The surface membranes are stabilized by various reagents in a hypotonic solution and are then removed intact or as large fragments with a Dounce homogenizer. The membranes are purified by differential centrifugation on solutions of sucrose or glycerol or on a column of fine glass beads. A trilaminar pattern can be seen in thin sections of the membrane in the electron microscope. Sufficient material can be conveniently obtained for chemical analyses.
Additional Material:
14 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040680308
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