ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The limitations of cosine methylene blue or basic fuchsin polypectate gels prompted further investigations to develop a selective pectate gel that could be used to enumerate or isolate gram-negative pectolytic bacteria even when they composed a minor portion of the microbial population.Crystal violet and brilliant green with bile proved the most effective of several selective agents studied. Of the two, crystal violet was much less inhibitory. Actidione, 10 mg per L added after sterilization, is helpful in the control of growth of molds and yeasts on this medium.The crystal violet medium has a productivity comparable to that of standard plate-count agar when pure cultures are used. It was successfully used to enumerate known populations of pectolytic gram-negative bacteria among mixed populations of other nonpectolytic gram-negative and -positive bacteria. It is also being used routinely for enumeration and isolation of pectolytic gram-negative bacteria from natural sources.Thus far, pectolytic bacteria belonging to 5 genera, including Aerobacter, Escherichia, Paracolobactrum, Aeromonas, and Achromobacter, have been isolated by use of the crystal violet gel.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1961.tb00809.x
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