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Insertion mutations in the control region of the Gal operon of E. coli (1972)

Saedler, H. ; Besemer, J. ; Kemper, B. ; [et al.]

Springer

Molecular genetics and genomics 115 (1972), S. 258-265

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ISSN: 1617-4623

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Galactose negative mutations are described which reduce the maximum expression of all three gal genes about 100-fold. The residual enzyme synthesis is not or only slightly inducible. These pleiotropic mutations map in the control region of the gal operon. No recombination is observed between these mutations. All mutants revert spontaneously to a Gal+ phenotype. In some mutations wildtype-like as well as constitutive revertants are obtained. The frequency of reversion can be increased by nitrosoguanidine (NG) in all mutants. The revertants, induced by this mutagen, are of a constitutive type.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00268889

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