ISSN:
1572-9699
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The plasma membrane ofHalobacterium salinarium, strain 1, has been isolated and characterised. A fraction containing cell envelope vesicles was isolated from a cell homogenate by centrifuging. A crude membrane fraction was obtained from the envelope fraction by dialysing it against distilled water, incubating with nucleases and centrifuging. A nucleotide-free purified membrane fraction, identified with the plasma membrane, was obtained by gel-filtration chromatography of the crude membrane fraction on Agarose. The nucleotide-free membrane-rich fraction contained all the cell lipid, including menaquinone and carotenoid, and cytochrome. No amino sugars could be detected. The action of the detergent, sodium dodecyl sulphate, on the nucleotide-free membrane-rich fraction broke up the membrane into smaller particles. The disaggregation occurred in at least two distinct steps. The disaggregated particles could be reaggregated to a fraction which resembled the original membrane by removing the detergent by dialysis or gel-filtration. A fraction which may be analogous to mitochondrial structural protein was isolated by ammonium sulphate fractionation of a preparation of the nucleotide-free membrane-rich fraction dissolved in a mixture of sodium deoxycholate, sodium cholate and sodium dodecyl sulphate. Protein fractions were separated from the nucleotide-free membrane-rich fraction during gel-filtration chromatography on Agarose in the presence of 6m urea.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02328118
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