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  • Life and Medical Sciences  (3)
  • 1970-1974  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    The structure of the hemocytes of Galleria mellonella (Lepidoptera) (1973)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 139 (1973), S. 105-123 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Four hemocyte types have been identified in the late last larval instar of Galleria mellonella. Plasmatocytoids are round to spindle shaped cells, 10-20 μ long and 5-10 μ wide. The cytoplasm contains no distinguishing inclusions. Golgi complexes, rough endoplasmic reticulum and free ribosomes are abundant. Granular hemocytes are oval shaped cells, 10-20 μ long and 5-10 μ wide. The granules, their most characteristic feature, have a diameter of 0.2 μ, a microtubular sub-structure, and are made up of acidic mucosubstances. Lipid droplets may be present in these cells at some stage of development. These cells appear to be phagocytic. Spherule cells are oval shaped, 15-20 μ long and 5-10 μ wide. The spherules, approximately 2 μ in diameter, have a highly ordered substructure and are made up of acidic mucosubstances. Oenocytoids are the largest cells, 20 by 40 μ. The cytoplasm contains mostly free ribosomes and microtubules.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051390107
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  • 2
    Electronic Resource
    Electronic Resource
    Genesis of the reproductive system of mosquitoes II. Male of Aedes stimulans (walker)Supported by a grant (GM-12612) from the National Institutes of Health followed by another (CC-00335) awarded by the Communicable Disease Center of the U.S. Public Health Service. (1970)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970), S. 329-357 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The imaginal male of mosquitoes bears a combination of organs and appendages that make it morphologically distinctive. Its reproductive organs produce sperm cells, convey and extrude them, provide accessory fluids, and insure copulation and insemination. In Aedes stimulans (Walker) these organs are derived from one of the two sets of primordia provided by the embryo. The second set of primordia is capable of producing the feminine reproductive system under unusual circumstances.Testes are derived from two compact ovoid masses of cells suspended in the hemocoel of abdominal segment 6. Each enlarges slowly throughout larval instars 1-3 and elongates very rapidly late in instar 4. Specialization of the cellular mass into sperm cells proceeds forward from the caudal end early in pupal life. From the beginning, a sheath of nutritive cells or fatbody encases each gonad, and no tracheation of the mass is evident although one small trachea sends branches to the encasing fatbody late in larval life.The efferent canal from each testis is derived from a tenuous filament extending caudally from each gonad to the venter of segment 9 and a small cluster of cells in the wall of the hemocoel on the ental surface of imaginal disc 9. Early in pupal life the filaments become the tubular vasa efferentia. The caudal clusters are primordial terminal parts of the lateral tract that become vasa deferentia, seminal vesicles and associated accessory glands. The ejaculatory canal comes from a short pouch derived from the median genital plate of disc 9.All external parts except the paraprocts are products of disc 9. The bilateral buds begin to proliferate in larval instar 4 and become the basistyles, dististyles and claspettes of the gonapophyses during pupal life. The phallosome is derived from the median genital plate.Primordia of a possible feminine reproductive system and cerci remain undifferentiated and disappear early in pupal life in the normal course of events. Primordia that were recognizable include those of ovaries, parts of lateral oviducts, median genital tract and cerci.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051310307
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  • 3
    Electronic Resource
    Electronic Resource
    Inhibition of marrow granulocytic colony formation by phytohemagglutininAided by grant T-559 from the American Cancer Society. (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 82 (1973), S. 277-283 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effect of phytohemagglutinin (PHA) on the growth and number of granulocytic colonies (GC) developing on agar from bone marrow and spleen cells of normal and erythroleukemic mice inoculated with Rauscher leukemogenic virus was studied. Equal number of marrow cells from erythroleukemic mice produced twice as many colonies as those from normal mice. The number of GC developing from either normal and leukemic spleen cells was only 20% to 25% of that arising from marrow cells. The number of cells within each colony was significantly larger in GC formed by myelogenous leukemic cells than those arising from normal cells even though they had similar morphologic features. The addition of 100 μg of PHA per 105 cells reduced the number of GC arising from normal and leukemic cells by 35% and 50%, respectively. Treatment with periodate which mainly inhibits its mitogenic activity, abolished the inhibitory effects of PHA on proliferation of granulocytic cells.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040820216
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