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  • Electron microscopy  (27)
  • nitrogen
  • Springer  (27)
  • PANGAEA
  • 1970-1974  (27)
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Keywords
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  • Springer  (27)
  • PANGAEA
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Inter- and intracisternal elements of the Golgi apparatus (1972)
    Springer
    Cell & tissue research 132 (1972), S. 365-380 
    Details
    ISSN: 1432-0878
    Keywords: Golgi apparatus ; Membranes ; Cross-bridges ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron opaque cross-bridge structures span the inter- and intracisternal spaces and provide membrane-to-membrane connections between adjacent cisternae of dictyosomes of pollen tubes ofClivia andLilium. Additionally, the classic intercisternal rods, characteristic of intercisternal regions near the maturing face of dictyosomes, are connected with the adjacent membranes through similar cross-bridge elements. We suggest that these structural links are responsible for maintaining the flattened appearance of the central parts of Golgi apparatus cisternac as well as for the coherence of cisternae within the stack. Observations on other plant (e.g. microsporocytes ofCanna) and animal cells (e.g. rodent liver and hepatoma cells, newt spermatocytes) show that such an array of membrane cross-links is a universal feature of Golgi apparatus architecture. The cross-bridges appear as part of the complex “zone of exclusion” which surrounds dictyosomes, entire Golgi apparatus and Golgi apparatus equivalents in a variety of cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02450714
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  • 2
    Electronic Resource
    Electronic Resource
    Elektronenmikroskopische Untersuchungen zur embryonalen und postembryonalen Genese der Eierstockzwischenzellen des Haushuhns (1973)
    Springer
    Cell & tissue research 136 (1973), S. 59-83 
    Details
    ISSN: 1432-0878
    Keywords: Interstitial cells, genesis ; Hen's ovary ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Für die Zwischenzellen des Hühnerovars gibt es embryonal und post-embryonal bindegewebige und epitheliale Quellen. Die bindegewebigen Mutterzellen sind Mesenchymzellen und Fibrozyten, die epithelialen Mutterzellen sind Wandepithelzellen der Markstranglakunen und Epoophorontubuli. Kriterien zur Unterscheidung dieser vierfachen Abstammungsmöglichkeiten der Zwischenzellen werden angegeben.
    Notes: Summary The interstitial cells of the hen's ovary have two sources: connective tissue mother cells, which are mesenchymal cells or fibrocytes, and epithelial mother cells, which are wall epithelial cells of the medullary cord lacunae or of the tubuli of the epophoron. Criteria are provided for differentiating this fourfold genesis of the interstitial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307680
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  • 3
    Electronic Resource
    Electronic Resource
    The ultrastructure of dentinogenesis and amelogenesis in rat molar tooth germs grown as organ cultures in vitro (1973)
    Springer
    Cell & tissue research 138 (1973), S. 171-186 
    Details
    ISSN: 1432-0878
    Keywords: Tooth germs ; Organ culture ; Differentiation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tooth germs from foetal rats of 17 days post-insemination were maintained in vitro for 12 days. Odontoblasts and ameloblasts differentiated and secreted their respective matrices in which mineralization occurred. The ultrastructure of the cells was qualitatively similar to that observed in normal development. Odontoblasts contained more lysosome-like bodies and were found to degenerate in some sites. Mantle dentine was formed but few von Korff fibres were observed. Calcospherites were rarely seen and the mineralizing front of dentine was predominantly linear, associated with numerous small early foci of mineral formation. Enamel showed prism formation associated with the Tomes' process of the ameloblast but some local disturbances in the pattern of enamel formation were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306606
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  • 4
    Electronic Resource
    Electronic Resource
    An analysis of some aspects of synaptosomal ultrastructure by the use of serial sections (1973)
    Springer
    Cell & tissue research 140 (1973), S. 481-496 
    Details
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Dense projections ; Complex vesicles ; Serial sections ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptosomes and synaptic junctions have been examined employing serial sections, with emphasis placed on four areas of investigation. 1. Starting from unequivocal synaptosomal profiles and tracing them through consecutive sections to the periphery of the synaptosomes, it is clear that vesicles are the one constant feature of the presynaptic terminal. In no instance was it possible to identify an empty membranous profile as synaptosomal. 2. Following a similar procedure it was found that the criteria required to predict the existence of a junctional region within a synaptosomal profile are: the accumulation of synaptic vesicles at one locus within its presynaptic component, and the presence of a postsynaptic profile characterized by two or more junctional features. 3. Serial sections of non-osmicated, PTA stained synaptic junctions confirm the regularity and orderliness of dense projection distribution along the length of the junction. 4. Complex vesicles can usually be followed in two and sometimes three adjacent sections, appearing either as intact vesicles or empty shells. Further observations confirmed that the latter profiles may be sections through the periphery of intact vesicles or through isolated shell fragments. They are more common in the latter form in unbuffered material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306675
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  • 5
    Electronic Resource
    Electronic Resource
    Post-coital depletion of dense-core vesicles from the external layer of the rabbit median eminence (1973)
    Springer
    Cell & tissue research 142 (1973), S. 27-36 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence (Rabbit) ; Dense-core vesicles depletion ; Releasing factors ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of mating on the depletion of osmiophilic material from dense-core vesicles (DCV) within the external layer of the rabbit median eminence (ME) were investigated. Two different populations of DCV were demonstrated within the ME external layer of the non-mated control animals. A plot of the diameters of these vesicles showed two peaks, one at 90–100 nm, and the other at 120–130 nm. These two populations of DCV were found in separate axons and axon terminals in contact with, or near the perivascular spaces of, portal capillaries. Within these axons and terminals an occasional membrane profile or “vesicle ghost” was also observed. The same two populations of DCV were evident in the ME external layer of animals that were sacrificed at 10 minutes post-coitus. In these experimental animals, the number and size of the smaller population of DCV were the same as those of the none-mated controls. However, there was an obvious decrease in the number of the large (120–130nm) DCV, together with a simultaneous marked increase in the number of “vesicle ghosts”. These ghosts had a mean diameter of 137±14 nm. On the basis of their size, it is suggested that the “vesicle ghosts” represent large DCV that have been depleted of their content following mating. Ultrastructural evidence for the depletion of osmiophilic material from the large DCV of mated animals is provided. According to previous writers the smaller DCV within the ME are aminergic, whereas the larger DCV may contain releasing factors (RF). Our findings provide further morphological evidence to support this hypothesis. Furthermore, RF and biogenic amines appear to be contained within separate nerve terminals in the rabbit ME.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306702
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of cycloheximide on the fine structure of corpus luteum in intact and hypophysectomized rats (1973)
    Springer
    Cell & tissue research 146 (1973), S. 223-235 
    Details
    ISSN: 1432-0878
    Keywords: Corpus luteum (Rat) ; Cycloheximide ; Hypophysectomy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In rats, one large intravenous dose of cycloheximide leads to extensive development of two types of membrane-formations in the cells of corpora lutea, within two hours. Both the laminated dense bodies (concentric layers of smooth membranes showing high electron density) and the tubular aggregates (tightly packed smooth tubules with diameter smaller than usual) exhibited obvious connections with endoplasmic reticulum membranes. The reorganization of tubular aggregates gave rise to “crystalloids” showing hexagonal symmetry. The crystalloids, being obviously unstable, were transformed into smooth fingerprints (concentric arrays of paired agranular membranes showing the same density as endoplasmic reticulum membranes). Hypophysectomy, performed 24 hours previously, moderated but did not totally abolish the development of membranous configurations. The described effect of cycloheximide is considered to represent cellular injury, probably due to membrane-denaturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307348
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  • 7
    Electronic Resource
    Electronic Resource
    Further observations on urethral chromaffin cells (1974)
    Springer
    Cell & tissue research 148 (1974), S. 499-504 
    Details
    ISSN: 1432-0878
    Keywords: Urethra (rat) ; Chromaffin cells ; Sensory neurons ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The urethra of the rat was examined by transmission and scanning electron microscopy. Under a transmission electron microscope flask-shaped chromaffin cells containing membrane-bound osmiophilic granules were seen to possess microvilli at their apical surfaces. The microvilli projected into large extracellular spaces which were apparently in continuity with the lumen of the urethra. Using scanning electron microscopy, a surface view of the lumen of the urethra was obtained. It showed a gently undulating surface with distinct intercellular boundaries. Scattered over the surface were numerous deep depressions between individual cells. These were thought to correspond with the large extracellular spaces into which microvilli had been seen to project. It is suggested that urethral chromaffin cells may “trigger” the afferent part of a reflex causing contraction of the urethral longitudinal muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221934
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  • 8
    Electronic Resource
    Electronic Resource
    Multinucleate spermatids in the mouse (1974)
    Springer
    Cell & tissue research 150 (1974), S. 323-329 
    Details
    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Mouse ; Multinucleate spermatids ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscope studies of the testes of five inbred and three outbred mouse strains show that the usual frequency of multinucleate spermatids is between one and two percent. In C57 BL/6J and A/Gr however it is higher (4–5%). The frequency of the shared acrosome condition, which would lead to the formation of an abnormal sperm, is 1.3 per thousand.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220140
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  • 9
    Electronic Resource
    Electronic Resource
    Spermatogenesis revisited (1973)
    Springer
    Cell & tissue research 138 (1973), S. 155-169 
    Details
    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Mouse ; Multinucleate spermatids ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This ultrastructural study confirms and extends the light microscope findings of Bryan (1971) concerning the presence and developmental fate of multinucleate spermatids. Four main classes of cells: uninucleate-individual, uninucleate-conjoined, multinucleate-conjoined, and multinucleate-individual, were identified along with a few instances of more complex syncytial organizations. When the respective nuclei in a given multinucleate are far enough apart, each develops autonomously but in synchrony with its neighbors. When nuclei are intimately associated, the “normal” pattern of spermiogenesis may be altered, giving rise to highly bizarre spermatozoa. Commonly, a single Golgi complex serves a pair of nuclei and gives rise to a “T-shaped” acrosome which binds the nuclei together. During the ensuing nuclear elongation phase, such units are invested by a single manchette. Pairs of axonemes within a common plasma membrane have also been encountered. These ultrastructural findings indicate that multinucleate spermatids are true components (not artifacts) of the seminiferous epithelium of normal animals. The presence of such cells and the unusual developmental consequences which can arise as a result of the multinucleate state must be taken into account when evaluating the course of spermatogenesis in cases of mutation- or chemically-induced infertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306605
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  • 10
    Electronic Resource
    Electronic Resource
    Über das fetale Gefäßsystem der reifen menschlichen Placenta (1973)
    Springer
    Cell & tissue research 139 (1973), S. 333-350 
    Details
    ISSN: 1432-0878
    Keywords: Placenta ; Human ; Blood vessels ; Endothelium ; Vascular muscle, Myoendothelial junctions ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Gefäße der Nabelschnur, Chorionplatte, Stammzotten und das Mikrozirkulationssystem des Zottenbaums der reifen menschlichen Plazenta wurden elektronenmikroskopisch untersucht. Die Endothel- und Muskelzellen besitzen auch am Ende der Gravidität alle morphologischen Kennzeichen voller Funktionsfähigkeit. Auffällig sind die zahlreichen myo-endothelialen Verzahnungen und die zahlreichen Kontakte der Muskelzellen untereinander. Sphinkterartige Einrichtungen werden nur an den Verzweigungen der Präkapillaren beobachtet. Gefäßnerven fehlen überall. Möglicherweise kann die Durchströmung der fetalen Placentagefäße durch autonome Kontraktion der Gefäßwände reguliert werden. — Alle Gefäße der Placenta sind elasticafrei. In den Nabelarterien sind elastische Systeme schwach ausgebildet. Eine Elastica interna wird nur in der Nabelvene gefunden. — Das Mikrozirkulationssystem in den Rami und Ramuli chorii sowie den Terminalzotten wird von allen bekannten Endstromeinheiten aufgebaut. Die englumigen Kapillarabschnitte befinden sich vor allem in unmittelbarer Nachbarschaft zum organellenreichen Syncytiophoblast, während die weitlumigen Abschnitte, die möglicherweise die venösen Kapillarstrecken sind, den Epithelplatten anliegen.
    Notes: Summary In the human placenta at term the blood vessels of the umbilical cord, chorionic plate and trunks and the microcirculation system of the placental lobes have been investigated. Also at the end of pregnancy the vascular endothelium and the smooth muscle cells exhibit all morphological criteria of full functional activity. The vessel wall is characterized by numerous myoendothelial junctions and many adjacent muscle cells being in close contact with one another. Sphincterlike structures can only be observed at the ramifications of the precapillaries. Vascular nerves are always absent. The blood circulation is perhaps regulated autonomously by means of the contractile vessel wall.—In all placental vessels a special tunica elastica never exists. In the umbilical arteries elastic systems are poorly developed. A genuine internal elastic layer only occurs in the corresponding vein.—In the region of the rami and ramuli chorii as well as in the single villi the microcirculation system consists of all types of terminal vessels. The capillaries with small diameter are preferentially situated close to the organellrich syncytiotrophoblast, whereas the distended segments, which may represent the venous capillaries, are in intimate contact with the epithelial plates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306590
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