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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical prospecting 18 (1970), S. 0 
    ISSN: 1365-2478
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Physics
    Notes: The paper deals with the early stages of development of a convenient form of electromagnetic induction method of sounding referred to as ‘Central Frequency Sounding’ and abbreviated as CFS. The method is introduced as a rapid and useful technique for investigation of shallow engineering and hydro-geological problems. Sets of theoretical two-layer master curves, suitable for interpretation of field data involving measurement of the vertical magnetic component of the field induced at the center of a loop placed on a two-layer earth, have been presented.The approximate but reasonably accurate solutions for a two-layer earth of any arbitrary resistivity contrast have been considered for the purpose and expressed in a form suitable for computation. The computed results have been presented in sets of curves useful for interpretation of field data.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 110 (1993), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Mutagenic effects of gamma rays and EMS using both single and combined dosages were examined in opium poppy (Papaver somniferum L.). For inducing both macro- and micro-mutations in the present study, combined mutagenic doses, especially 5 kR + 0.4 % EMS appeared to be greatly superior to all single doses of the two mutagens: gamma rays and EMS. The lowest dose of combined mutagen (5 kR + 0.2 % EMS) was about twenty times more effective than the most effective dose (15 kR) of gamma rays and twice as effective as the most effective concentration (0.4 %) of EMS. The combined dose 5 kR + 0.4 % EMS enhanced morphine content (%) to 0.98 as against 0.64 in the control. In accordance with earlier findings (GAUL 1964) the study revealed a correlation between induced macro- and micro-mutations.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 43 (1978), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Acid inorganic pyrophosphatase on the one hand, and Mg2+-dependent alkaline inorganic pyrophosphatase and Zn2+-dependent acid inorganic pyrophosphatase on the other hand showed opposite trends in their activities in rice (Oryza sativa L. cv. Ratna) seedlings grown in dark and sun. The opposite trends in their activities were also noted in rice seedlings grown from gamma-irradiated seeds and in detached rice leaves floated on water in dark. The ratios of Mg2+ dependent alkaline inorganic pyrophosphatase/acid inorganic pyrophosphatase and Zn2+-dependent acid inorganic pyrophosphatase/acid inorganic pyrophosphatase changed significantly in response to the above physical treatments, but the ratio of Mg2+ dependent alkaline inorganic pyrophosphatase/Zn2+ dependent acid inorganic pyrophosphatase remained relatively stable. The conclusion is that Zn2+-dependent acid inorganic pyrophosphatase activity is the same as that of Mg2+-dependent alkaline inorganic pyrophosphatase and is different from that of acid inorganic pyrophosphatase, which requires no metal ion for activity. The acid and alkaline inorganic pyrophosphatase activities are due to separate enzyme proteins.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 15 (1996), S. 0 
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract A 277-bp long DNA fragment, Ba813, was isolated from an avirulent Bacillus anthracis strain 7700 genomic library. Two oligonucleotides derived from the Ba813 sequence were used as primers in polymerase chain reaction tests on genomic DNA from 28 Bacillus anthracis and from 33 heterologous bacteria strains. A specific, 152-bp long DNA fragment was amplified only when Bacillus anthracis DNA was used as the target. The amplified product was analysed by non-radioactive sandwich hybridisation in microtiter plates using two oligonucleotides. The capture oligonucleotide C1 was covalently linked onto aminated wells of microtiter plates. The detection oligonucleotide D3 was labelled with biotine. The hybrid molecules were detected by avidine conjugated with alkaline phosphatase and chromogenic substrate. Amplification of Ba813 sequence may provide the basis for rapid and reliable assay for the detection and identification of Bacillus anthracis.
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  • 5
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isolates, and simultaneous confirmation of the species identity independent of plasmid content. The assay amplifies lef, cya, pag (pXO1) and cap (pXO2) genes, and a B. anthracis specific chromosomal marker, giving an easy-to-read profile. This system unambiguously identified virulent (pXO1+/2+) and avirulent (pXO1+/2−, pXO1−/2+ and pXO1−/2−) strains of B. anthracis and distinguished ‘anthrax-like’ strains from other B. cereus group bacteria.
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