ISSN:
1432-2048
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Nitrate reductase (EC 1.6.6.1-2) purified from nitrogen-deficient cells of Ankistrodesmus braunii has the same characteristics previously described for the enzyme from Chlorella fusca. Nitrogen-deficient cells were chosen as a source for nitrate reductase because of a pronounced rise of enzymatic activity after about 20 days of growth, which surpassed even the specific activity present in normal cells. This nitrate reductase exhibits a twofold specificity towards NADH and NADPH which shows a constant ratio during enzyme purification and cannot be separated by gelfiltration or density gradient centrifugation. By growing Ankistrodesmus in the presence of radioactive 55Fe, the incorporation of this metal into the purified enzyme could be demonstrated. A scheme is presented for the enzymatic mechanism of nitrate reduction in green algae.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00386893
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