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  • Electron microscopy  (31)
  • Light and electron microscopy  (7)
  • Springer  (38)
  • Cambridge University Press
  • 1970-1974  (38)
  • 1945-1949
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Keywords
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  • Springer  (38)
  • Cambridge University Press
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Post-coital depletion of dense-core vesicles from the external layer of the rabbit median eminence (1973)
    Springer
    Cell & tissue research 142 (1973), S. 27-36 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence (Rabbit) ; Dense-core vesicles depletion ; Releasing factors ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of mating on the depletion of osmiophilic material from dense-core vesicles (DCV) within the external layer of the rabbit median eminence (ME) were investigated. Two different populations of DCV were demonstrated within the ME external layer of the non-mated control animals. A plot of the diameters of these vesicles showed two peaks, one at 90–100 nm, and the other at 120–130 nm. These two populations of DCV were found in separate axons and axon terminals in contact with, or near the perivascular spaces of, portal capillaries. Within these axons and terminals an occasional membrane profile or “vesicle ghost” was also observed. The same two populations of DCV were evident in the ME external layer of animals that were sacrificed at 10 minutes post-coitus. In these experimental animals, the number and size of the smaller population of DCV were the same as those of the none-mated controls. However, there was an obvious decrease in the number of the large (120–130nm) DCV, together with a simultaneous marked increase in the number of “vesicle ghosts”. These ghosts had a mean diameter of 137±14 nm. On the basis of their size, it is suggested that the “vesicle ghosts” represent large DCV that have been depleted of their content following mating. Ultrastructural evidence for the depletion of osmiophilic material from the large DCV of mated animals is provided. According to previous writers the smaller DCV within the ME are aminergic, whereas the larger DCV may contain releasing factors (RF). Our findings provide further morphological evidence to support this hypothesis. Furthermore, RF and biogenic amines appear to be contained within separate nerve terminals in the rabbit ME.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306702
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on the acetylcholinesterase (ache)-positive and -negative autonomic axons supplying smooth muscle in the normal and 6-hydroxydopamine (6-OHDA)treated rat iris (1973)
    Springer
    Cell & tissue research 138 (1973), S. 211-222 
    Details
    ISSN: 1432-0878
    Keywords: Iris ; Muscle, Nerves ; Light and electron microscopy ; Acetylcholinesterase ; 6-Hydroxydopamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenergic and cholinergic innervation to the rat iris has been studied at a light and electron microscopic level. Catecholamine fluorescence histochemistry showed adrenergic nerves to be present in both the dilatator and the constrictor pupillae regions. At a fine structural level the terminal innervation of the iris was studied and criteria for the differentiation between presumptive adrenergic and presumptive cholinergic axon terminals were examined. To aid this examination presumptive adrenergic axons were either labelled with the “false” adrenergic transmitter, 5-hydroxydopamine, or chemical sympathectomy performed using 6-hydroxydopamine. The value of using acetylcholinesterase staining as a marker for cholinergic nerve terminals was also studied. Results showed a mixed adrenergic/cholinergic innervation to the dilatator pupillae. In the constrictor pupillae an exclusively cholinergic innervation was found although adrenergic and cholinergic nerves were found supplying the blood vessels and at the dilatator-constrictor interface. These findings are discussed with regard to innervation-function relationships in the iris.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306608
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  • 3
    Electronic Resource
    Electronic Resource
    Cloned pigmented retinal cells; the affects of cytochalasin B on ultrastructure and behavior (1972)
    Springer
    Cell & tissue research 130 (1972), S. 135-151 
    Details
    ISSN: 1432-0878
    Keywords: Pigmented retina ; Cytochalasin B ; Clonal culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Three-week-old clones of pigmented retinal cells can be divided into four morphologically distinct regions: an outer zone of flattened cells, the squamous zone; a ring of rounded to spindle shaped cells which pile up and secrete large amounts of mucopolysaccharide, the stratified zone; a region of lightly pigmented cells, the intermediate zone; and the center consisting of a single layer of heavily pigmented cuboidal cells, the pigmented cuboidal zone. The ultrastructure of the cells in the pigmented cuboidal zone and the epithelial cells of eleven-day-old embryonic pigmented retina is similar. Both cell types are cuboidal in profile, rest on a typical basal lamina, interdigitate with adjoining cells at their bases, and have apical surface protrusions. The embryonic epithelial cells and cells of the two innermost zones of the clones have a web of thin filaments (40–70 Å in diameter), just beneath the apical protrusions. Skeins of these filaments are often interconnected by discrete dense bodies. Near the zonulae adhaerentes bundles of filaments are embedded in peripheral dense material and extend around the periphery of the cell. After treatment with low concentrations (1 μg/ml) of cytochalasin B (CCB) the retiform layer of filaments beneath the apical protrusions is dispersed, but the peripheral filaments remain intact. Cells treated in this manner retain their polygonal shape but their surfaces are altered. Higher concentrations of CCB (10 μg/ml) disperse the filaments and disrupt the junctional complexes. The cells lose their surface protrusions and become more spherical. Thick filaments and microtubules are unaffected by CCB. Time-lapse cinematography reveals active movements of apical protrusions and focal contractions of cells of the intermediate zone. These movements are blocked by CCB. We infer that the apical filaments are implicated in the activity of the apical protrusions and at least partially responsible for the maintenance of the polygonal shape. Contraction of these arrays of filaments may be responsible for the focal contractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306953
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  • 4
    Electronic Resource
    Electronic Resource
    Bone formation at a ceramic implant interface (1971)
    Springer
    Calcified tissue international 8 (1971), S. 165-171 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Ceramic ; Tetracycline ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Un implant céramique non poreux est testé au niveau du fémur de rat en ce qui concerne son adhésivité à l'os. Un certain nombre de techniques morphologiques sont utilisées pour examiner le rapport entre l'implant et l'os néoformé. La microscopie électronique par transmission et la microscopie par fluorescence après marquage à la tétracycline ont donné les meilleurs résultats. Un rapport étroit entre l'os minéralisé et la céramique a été noté en microscopie électronique. Par marquage à la tétracycline, il semble que l'implant puisse stimuler la formation osseuse.
    Abstract: Zusammenfassung Ein unporöses keramisches Implantat in Rattenfemora wurde auf seine Fähigkeit geprüft, sich mit Knochen zu binden. Eine Anzahl morphologischer Techniken wurde verwendet, um die Beziehung zwischen den Oberflächen von Implantat und neuem Knochen zu untersuchen. Transmissions-Elektronenmikroskopie und Fluoreszenzmikroskopie nach Tetracyclinmarkierung waren die erfolgreichsten Techniken. Eine enge Beziehung zwischen mineralisiertem Knochen und dem Keramikimplantat konnte mit der Transmissions-Elektronenmikroskopie nachgewiesen werden. Das Aussehen der Tetracyclinmarkierung im keramischen Implantat deutet darauf hin, daß dieses wahrscheinlich die Fähighkeit hat, Knochenbildung zu erhöhen.
    Notes: Abstract A nonporous ceramic implant in rat femora was evaluated as to its ability to bond to bone. A number of morphologic techniques were utilized to examine the interfacial relationship of the implant to new bone. Transmission electron microscopy and fluorescence microscopy after tetracycline labelling were the most successful techniques. An intimate relationship between mineralized bone and the ceramic was demonstrated by transmission electron microscopy. The appearance of tetracycline labelling at the ceramic interface indicates that the implant may have capacity to enhance bone formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010133
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  • 5
    Electronic Resource
    Electronic Resource
    Fine structure of the neural sheath, glia and neurons in the brain of the housefly,Musca domestica (1972)
    Springer
    Cell & tissue research 135 (1972), S. 449-459 
    Details
    ISSN: 1432-0878
    Keywords: Insect brain ; Neurons ; Glia ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the neural sheath, glial cells and nerve cells in the brain of adult male houseflies is described. The neural sheath is composed of neural lamella and perineurium. The neural lamella consists of an external lamina and collagen-like fibrils which are embedded in an amorphous matrix. The perineurial cells form a continuous layer around the brain. On their inner surface, perineurial cells form junctional complexes with glial cell processes. A cortical cellular layer composed of neurons and glial cells surrounds the centrally located neuropil. Three types of glial cells are identified. Glial cells differ in size and in relative development and distribution of organelles. Thin processes of glioplasm completely surround the cell bodies of the neurons. Five types of neurons are described. Most of the neurons are monopolar, a few are bipolar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583429
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  • 6
    Electronic Resource
    Electronic Resource
    Comparative ultrastructural study of normal and grafted skin in the frog,Rana pipiens, with special reference to neuroepithelial connections (1973)
    Springer
    Cell & tissue research 139 (1973), S. 451-462 
    Details
    ISSN: 1432-0878
    Keywords: Skin ; Anurans ; Regional differences in grafts ; Neuroepithelial relationships ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Recent investigations have suggested specific differences in back and belly skin in anurans which appear to influence the quality of reflex responses obtained from various areas of the animals body. The present investigation represents a comparative morphological study of back and belly skin in control and skin-graftedRana pipiens, with special regard to the neuroepithelial relationships. A distinct difference in pigmentation of back and belly skin was observed. Intra-epithelial Merkel cells were present in all skin samples studied. The origins of the numerous unclassifiable cells in the Merkel cell region are discussed in relation to a presumed coordinating function of the Merkel cell during epithelial differentiation. Epitheliomesenchymal interactions were observed in the richly innvervated dermal regions. Two types of morphologically different intra-epithelial nerve endings were observed. These observations are discussed in relation to earlier observations on vertebrate skin and in relation to misdirected reflex responses obtained in skin-grafted anurans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02028387
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  • 7
    Electronic Resource
    Electronic Resource
    An analysis of some aspects of synaptosomal ultrastructure by the use of serial sections (1973)
    Springer
    Cell & tissue research 140 (1973), S. 481-496 
    Details
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Dense projections ; Complex vesicles ; Serial sections ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptosomes and synaptic junctions have been examined employing serial sections, with emphasis placed on four areas of investigation. 1. Starting from unequivocal synaptosomal profiles and tracing them through consecutive sections to the periphery of the synaptosomes, it is clear that vesicles are the one constant feature of the presynaptic terminal. In no instance was it possible to identify an empty membranous profile as synaptosomal. 2. Following a similar procedure it was found that the criteria required to predict the existence of a junctional region within a synaptosomal profile are: the accumulation of synaptic vesicles at one locus within its presynaptic component, and the presence of a postsynaptic profile characterized by two or more junctional features. 3. Serial sections of non-osmicated, PTA stained synaptic junctions confirm the regularity and orderliness of dense projection distribution along the length of the junction. 4. Complex vesicles can usually be followed in two and sometimes three adjacent sections, appearing either as intact vesicles or empty shells. Further observations confirmed that the latter profiles may be sections through the periphery of intact vesicles or through isolated shell fragments. They are more common in the latter form in unbuffered material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306675
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructure of leydig cell paracrystalline inclusions, possibly related to reinke crystals, in the normal human testis (1973)
    Springer
    Cell & tissue research 142 (1973), S. 13-26 
    Details
    ISSN: 1432-0878
    Keywords: Paracrystals ; Reinke crystals ; Leydig cells ; Normal human testis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary One of two types of paracrystalline inclusions, other than Reinke crystals, was encountered in the Leydig cells of the normal mature testes of 9 out of 10 patients. The basic ultrastructure of these formations was that of parallel arrays of fine filaments (8 cases) or tubules (1 case). The filamentous structures, designated as type A paracrystals, consisted of fascicles of parallel rows of 4 or 5 dense fibrils. The latter typically contained dense granules or banded striations spaced at regular intervals. In 4 cases the inclusions were found in the cytoplasm while in 4 others they were confined to the nuclei. The tubular type of paracrystal, type B, was observed in only one patient. Here, groups of closely packed tubular inclusions occupied large portions of cytoplasm. The tubular walls were composed of closely applied, small, circular profiles. In no instance were paracrystals and Reinke crystals encountered in the same cell. A possible role of the former as precursors of the latter is discussed but there is no strong supporting evidence for this despite certain suggestive similarities in unit and subunit measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306701
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  • 9
    Electronic Resource
    Electronic Resource
    A comparison of the fine structure of thoracic and abdominal interganglionic connectives in the newly hatched and adult stick insect, Carausius morosus Br. (1973)
    Springer
    Cell & tissue research 145 (1973), S. 299-309 
    Details
    ISSN: 1432-0878
    Keywords: Nervous system ; Stick insect ; Blood-brain barrier ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fat body sheath which surrounds the ventral nerve cord of the adult stick insect, Carausius morosus, is absent in the hatchling. Since the haemolymph sodium ion compositions of the two stages are similar, it is suggested that the fat body sheath is not a site of a blood-brain barrier dealing with extra-axonal regulation of sodium. Similarities of ultrastructure in connectives of adults and hatchlings are demonstrated, and tight junctions are shown to occur in perineural cells of both stages. The possible significance of this to the blood-brain barrier of this insect is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307160
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  • 10
    Electronic Resource
    Electronic Resource
    Further observations on urethral chromaffin cells (1974)
    Springer
    Cell & tissue research 148 (1974), S. 499-504 
    Details
    ISSN: 1432-0878
    Keywords: Urethra (rat) ; Chromaffin cells ; Sensory neurons ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The urethra of the rat was examined by transmission and scanning electron microscopy. Under a transmission electron microscope flask-shaped chromaffin cells containing membrane-bound osmiophilic granules were seen to possess microvilli at their apical surfaces. The microvilli projected into large extracellular spaces which were apparently in continuity with the lumen of the urethra. Using scanning electron microscopy, a surface view of the lumen of the urethra was obtained. It showed a gently undulating surface with distinct intercellular boundaries. Scattered over the surface were numerous deep depressions between individual cells. These were thought to correspond with the large extracellular spaces into which microvilli had been seen to project. It is suggested that urethral chromaffin cells may “trigger” the afferent part of a reflex causing contraction of the urethral longitudinal muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221934
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