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  • 1975-1979  (2)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Control of cI gene expression in bacteriophage λimm 434, studied in an immunity/trp fusion made in vitro (1976)
    Springer
    Molecular genetics and genomics 146 (1976), S. 191-198 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The trp genes of a λimm 434 trp-transducing phage have been fused to the immunity region by deletion, in vitro, of the DNA between two targets for the restriction enzyme R.EcoRI. The resulting phage has been used to study the control of expression of the cI gene in vivo. The constitutive rate of expression of the cI gene is between 2 and 5% of the maximally stimulated rate. The products of the cII and cIII genes enhance expression of cI on infection of a sensitive host. The requirement for the cII product is more stringent than that for the cIII protein. The phage 434 repressor present in a 434-immune cell stimulates the rate of cI expression from a superinfecting homoimmune phage about fifteen-fold. This result strongly suggests that repressor stimulates its own synthesis by a direct effect on transcription of the cI gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268088
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro insertion of the λ attachment site into the plasmid RP4 (1979)
    Springer
    Molecular genetics and genomics 177 (1979), S. 163-168 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The region of the phage lambda chromosome containing the attachment site (P · P′) and the genes int and xis, excised by the action of endonuclease R.EcoRI, has been inserted into the unique site for that enzyme on the promiscuous conjugative plasmid, RP4, generating the recombinant plasmid RP4attλ. Transformants containing the hybrid plasmid were recognised by their ability to allow efficient lysogenization by phage λb2 (Weil and Signer, 1968; Echols et al., 1968) containing the mutant attachment site Δ · P′. The construction and properties of the hybrid plasmid RP4attλ are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267266
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    Paper (German National Licenses)
    Fulltext
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