ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary As continuous analytical method to the enzymatic conversion of glucose to gluconic acid the well-known method of polarimetry is applied. In this case a simple polarimetric analysis is only possible by setting the pH to 10. Because the pH optimum of the reaction is at pH 6 this is carried out in the outlet of a continuous reactor before the product stream reaches the flow through cuvette of the polarimeter. The variability coefficient of the polarimetric method is 0.8 % in comparison to 1.6 % of the classical photometric method. The high precision and the high time resolution of the polarimetric method makes it applicable to continuous operation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00928426
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