Publication Date:
1979-03-23
Description:
Cultured skin fibroblasts from subjects with cystic fibrosis exhibited normal population doubling times in early passages. After about 13 cumulative population doublings, cystic fibrosis lines doubled more slowly than controls and ceased doubling after about 19 weekly passages. Control lines continued doubling for 27 passages. The premature senescence noted in cells from subjects with cystic fibrosis reconciles controversial observations of cell doubling reported in the literature. Data presented here demonstrate that experiments with cystic fibrosis cells in late passage may generate misleading results since differences from control lines may be ascribed to generalized senile changes rather than to specific results of the cystic fibrosis genotype.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shapiro, B L -- Lam, L F -- Fast, L H -- New York, N.Y. -- Science. 1979 Mar 23;203(4386):1251-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/424752" target="_blank"〉PubMed〈/a〉
Keywords:
Adolescent
;
Adult
;
Aging
;
Cell Division
;
*Cell Survival
;
Cells, Cultured
;
Child
;
Cystic Fibrosis/*pathology
;
DNA/biosynthesis
;
Female
;
Humans
;
Male
;
Skin/pathology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink