ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1975-1979  (2)
Collection
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of the minimal fragment required for autonomous replication (“Basic replicon”) of a copy mutant (pKN102) of the antibiotic resistance factor R1 (1978)
    Springer
    Molecular genetics and genomics 162 (1978), S. 51-57 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mini plasmids deriving from pKN102, a copy mutant of the antibiotic resistance factor R1drd-19 of E. coli, share a common DNA sequence of 2.6 kb, which carries the minimal functions for autonomous replication. By cloning of two PstI fragments of this region it could be demonstrated that the “basic replicon” is a DNA segment not larger than 1.8 kb, which carries the origin of replication and the genetic information for at least two proteins. Protein F (MW=11.000 dalton) seems to be synthesed in larger amounts in minicells of E. coli than protein C (20.000 dalton). Plasmids containing this isolated replicon of R1 are completely compatible with the parental plasmid R1drd-19.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333850
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The nucleotide sequence of a DNA fragment from the replication origin of the antibiotic resistance factor R1drd19 (1979)
    Springer
    Molecular genetics and genomics 171 (1979), S. 277-285 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The recombinant plasmid pRK101 contains a DNA fragment which carries the complete replication origin of the antibiotic resistance factor R1drd-19 inserted into the vector plasmid pBR322. In a spontaneously arising mutant of this plasmid (pRK 103) a deletion of about 215 base pairs (bp) has been detected by heteroduplex analysis and mapping with restriction endonucleases. Essential parts of the replication origin must be located in the deleted sequence. The deletion mutant pRK103, in contrast to its parent plasmid pRK101 is not replicated under the control of the R1 replicon, even when the R1 factor or copy mutants of it are present within the same cell. These latter plasmids can complement a plasmid-specific protein not coded by pRK101 but essential for R1-directed replication. The nucleotide sequence of a 252 bp HpaII fragment covering about 170–200 bp of the deletion was determined. This piece of DNA is rich in G and C and contains a series of small palindromes, symmetrically arranged repeated sequences and short selfcomplementary structures which may be of significance for the initiation of the DNA replication. The possibility that the sequenced DNA fragment comprises a major part of the replication origin of R1drd-19 is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267582
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...