ISSN:
1432-1793
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An Antarctic psychrophilic marine Vibrio species was isolated having the inducible ability to accumulate nonmetabolizable thiomethyl-14C-β-galactopyranoside (14C-TMG) through a galactose permease system. Induction of 14C-TMG uptake was found to have a salinity requirement which was higher than that required for uptake. At the optimum salinity, galactose and fucose were the primary inducers. Lactose produced a comparable induction but only at higher concentrations, whereas glucose did not cause induction. The initial rate of 14C-TMG uptake exhibited saturation kinetics with an apparent Km value of 4.8 x 10−6M. An amino acid, in addition to the inducer, was required for induction which could not be replaced by glycerol or galactose. Evidence is presented which indicates that the uptake of 14C-TMG is energy-dependent and that nutrient availability is more important than salinity for induction and uptake under conditions which would normally be found in the oceanic environment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00390724
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