ISSN:
1573-8221
Keywords:
experimental allergic encephalomyelitis
;
antilymphocytic serum
;
immunodepression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Experimental allergic encephalomyelitis (EAE) was induced in guinea pigs by sensitization with myelin of homologous or heterologous (rabbit) brain in Freund's complete adjuvant. In the early stages before or after sensitization the animals were given subcutaneous injections of antilymphocytic serum (ALS), obtained by immunizing rabbits with lymphocytes from guinea pig lymph nodes, daily for 3–6 days. The ALS had a marked inhibitory action on the development of neurological manifestations of EAE and histological changes, namely demyelination and perivascular cell infiltration in the lumbosacral and cervical segments of the spinal cord. ALS was most effective when injected starting from the third to fifth day before sensitization or from the day of induction of EAE. A later start of the injections (from the seventh or 12th day after sensitization) was accompanied by increased morbidity in the groups. A decrease in morbidity correlated with a decrease in the number of positive skin tests to intradermal injection of homologous myelin. The production of complement-fixing antibodies against heterologous myelin was not reduced in the animals receiving ALS. This points to the participation of cellular factors in the development of the pathological process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00789868
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