Publication Date:
1979-02-16
Description:
Exposure of conjugating Tetrahymena to a hyperosmotic shock blocks the exchange of gametic nuclei and produces self-fertilized exconjugants that are homozygous for their whole genome. Cells are sensitive to this induction during a brief period after meiosis. The high efficiency of the treatment and the fertility of the progeny make this a useful method for the isolation of induced recessive mutations and enhances the value of Tetrahymena as an animal-cell model system in which genetic dissection is practical. The sharp peak of sensitivity is useful in the study of those cellular mechanisms responsible for the independent handling of several functionally distinct nuclei during conjugation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Orias, E -- Hamilton, E P -- Flacks, M -- New York, N.Y. -- Science. 1979 Feb 16;203(4381):660-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/760210" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Nucleus/physiology
;
Conjugation, Genetic
;
Genes, Recessive
;
Homozygote
;
Mutation
;
Osmotic Pressure
;
Tetrahymena/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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