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  • Life and Medical Sciences  (5)
  • General Chemistry  (2)
  • ddc:330
  • 1975-1979  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Induced alteration in uptake properties of Tetrahymena and its association with the development of mating competency (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 92 (1977), S. 77-90 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The rate of uridine uptake in Tetrahymena declines by an order of magnitude by two hours after shiftdown to a non-nutrient buffer. This alteration in uptake properties cannot be accounted for by an increase in the intracellular pool of uridine, an increase in apparent Km for uptake or a decline in the rate in which uridine is processed intracellularly.It is argued that the decrease in uridine uptake is due to a reduction in numbers of functional transport molecules exposed at the cell surface and is a reflection of a developmentally related cell surface transformation.In addition, the putative decline in functional transport molecules cannot be entirely explained by metabolic turnover of these molecules in the absence of replacement, nor does it require the synthesis of new protein. We discuss the possibility that a shift in equilibrium between accessible and inaccessible transporters is operating.Finally, a close correlation between conditions which elicit the transport alteration and those which allow the development of mating competency suggests that the two phenomena may be coordinately regulated.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040920110
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  • 2
    Electronic Resource
    Electronic Resource
    Phenotypic characterization of ouabain-resistant Aedes albopictus cells (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 101 (1979), S. 515-521 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The phenotype of a ouabain-resistant Aedes albopictus cell line has been partially characterized. Treatment of ouabain-sensitive cells with 0.005-1.0 mM ouabain resulted in an 80% reduction in the uptake of 86rubidium (86Rb+), an ion with an affinity for the K+ pump binding site; ouabain-resistant cells showed only a 40% reduction with 1.0 mM ouabain. When ouabain-sensitive cells were incubated in the presence of ouabain (0.1 mM) for one and one-half to three hours, the molar ratio of intracellular Na+/K+ rose from 0.2 to 4.2. In ouabain-resistant cells, a similar treatment had very little effect. Based on [3H] ouabain-binding studies, ouabain-resistant cells were estimated to have 60% fewer binding sites per cell than ouabain-sensitive cells. The spontaneous mutation rate from ouabain sensitivity to ouabain resistance was calculated to be 1-6 X 10-8 mutations/cell/generation, a value similar to that reported for mammalian cells at the analogous locus.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041010317
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  • 3
    Electronic Resource
    Electronic Resource
    Initiation of 3T3 fibroblast cell division by epidermal growth factor (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 86 (1975), S. 593-598 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Epidermal Growth Factor (EGF) at concentrations of 10-9 to 10-10 M initiates cell division in both confluent and low density non-dividing 3T3 cells. Four days after addition of EGF to confluent or low density non-dividing 3T3 cells there is a 2- and 5-fold increase, respectively, in cell number.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040860504
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  • 4
    Electronic Resource
    Electronic Resource
    Development of a culture medium for growing xenopus laevis oocytes (1978)
    New York, NY : Wiley-Blackwell
    Gamete Research 1 (1978), S. 269-280 
    Details
    ISSN: 0148-7280
    Keywords: oocyte growth ; oocyte culture ; vitellogenin ; insulin ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A defined medium for growing defolliculated Xenopus laevis oocytes was developed by monitoring vitellogenin incorporation. Optimum conditions were achieved by use of 50% Liebovitz L-15 medium supplemented with 1 mM L-glutamine, 15 mM Hepes, 5 mg/ml vitellogenin, and 1 μg/ml insulin (final pH = 7.8). Stage IV/V oocytes remained morphologically normal in this medium for at least two weeks and grew at an average rate of 0.25 mm3 per week. This is the first time defolliculated Xenopus laevis oocytes have been grown in vitro.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120010307
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  • 5
    Electronic Resource
    Electronic Resource
    Terminal deoxynucleotidyl transferase in rooster sperm (1978)
    New York, NY : Wiley-Blackwell
    Gamete Research 1 (1978), S. 227-233 
    Details
    ISSN: 0148-7280
    Keywords: sperm ; terminal deoxynucleotidyl transferase ; DNA ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A 165,000g pellet fraction, isolated from lysed rooster spermatozoa, was shown to contain a deoxynucleotide polymerizing activity which banded in sucrose at a buoyant density of 1.15gm/ml. The solubilized enzyme, partially purified by gel filtration, was estimated to have a molecular weight of 33,000 and utilized dT10 or dG10 as exogenous primers in template-independent reactions sensitive to either sodium pyrophosphate or ethylenediaminetetra- cetic acid. Pyrimidine nucleotides (dTTP) were preferentially polymerized in the presence of Co++, while purine nucleotides (dGTP) were the preferred substrate when Mn++ was the divalent cation. These properties are all consistent with those of terminal deoxynucleotidyl transferase. An enzyme with similar properties was also obtained by subjecting a rooster sperm lysate to the procedure utilized to isolate terminal deoxynucleotidyl transferase from calf thymus. It is hypothesized that the enzyme may be involved in the regulation of gene expression during embryogenesis.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120010302
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  • 6
    Electronic Resource
    Electronic Resource
    Dreiphasen-Katalyse (1979)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 91 (1979), S. 464-472 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die vor kurzem eingeführte Dreiphasen-Katalyse (TPC) ist eine spezielle Form der heterogenen Katalyse: Der Katalysator, das Substrat und das Reagens liegen jeweils in gesonderten Phasen vor. Auf dieser Grundlage wurden neue Syntheseverfahren entwickelt, bei denen Reaktionen in wäßrig-organischen Zweiphasensystemen durch feste Katalysatoren in Gang gebracht werden. Obwohl die TPC erst am Anfang ihrer Entwicklung steht, läßt sich schon heute eine große Anwendungsbreite absehen. Vom mechanistischen Verständnis der sehr komplexen katalytischen Systeme ist man aber noch weit entfernt; um die Beziehungen zwischen TPC, Phasentransfer-Katalyse, micellarer Katalyse und Grenzflächenkatalyse zu klären, sind eingehende Untersuchungen erforderlich.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19790910605
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  • 7
    Electronic Resource
    Electronic Resource
    Triphase Catalysis [New synthetic methods (27)] (1979)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 18 (1979), S. 421-429 
    Details
    ISSN: 0570-0833
    Keywords: Triphase catalysis ; Catalysis ; Synthetic methods ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Triphase catalysis (TC) has recently been introduced as a unique form of heterogeneous catalysis in which the catalyst and each of a pair of reactants are located in separate phases. Based on this concept, new synthetic methods have been developed for aqueous phase-organic phase reactions using a solid phase catalyst. Although it is only at an early stage of development, TC shows considerable potential for practical use. Our mechanistic understanding of these highly complex catalytic systems is at present very limited and detailed examination will be required before their relationship to phase-transfer, micellar, and interfacial catalysis becomes clear.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.197904213
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