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  • Cytochemistry  (2)
  • hemoglobin  (2)
  • Springer  (4)
  • 1975-1979  (4)
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  • Springer  (4)
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  • 1975-1979  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    The hemoglobins of Artemia salina. IV. A model for genetic control of hemoglobin 1, hemoglobin 2, and hemoglobin X (1977)
    Springer
    Biochemical genetics 15 (1977), S. 409-422 
    Details
    ISSN: 1573-4927
    Keywords: crustacean ; hemoglobin ; polyacrylamide electrophoresis ; Artemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two loci account for all genetic variation resulting in difference in electrophoretic mobility in three hemoglobins (Hb1, Hb2, and HbX) in the hemolymph of the brine shrimp. Four α alleles and nine β alleles have been studied. In shrimps of all genotypes and in electrophoresis in media with varying degrees of molecular sieving, Hb2 is approximately equidistant from Hb1 and HbX. A shrimp heterozygous at both loci has a three-banded Hb1, a four-banded Hb2, and a three-banded HbX. We conclude that Hb2 contains n α-polypeptides and n β-polypeptides. Hb1 contains 2n α-polypeptides. HbX contains 2n β-polypeptides. During electrophoresis, the three native hemoglobins undergo reversible dissociation to n subunits. Subunits with the same charge reassemble to migrate as molecules of the same size as the native molecules. Although there is no evidence for an additional polypeptide in the three hemoglobins, we cannot exclude such a possibility. If it exists, it is under three constraints: (1) it must be present in equal amounts in each of the three hemoglobins; (2) it must have the same molecular weight as the α- and β-polypeptides; and (3) it must be free of genetic variation (detectable by electrophoresis).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520187
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  • 2
    Electronic Resource
    Electronic Resource
    The hemoglobins of Artemia salina. V. Genetic variation in hemoglobin 1, hemoglobin 2, and hemoglobin 3 (1977)
    Springer
    Biochemical genetics 15 (1977), S. 423-437 
    Details
    ISSN: 1573-4927
    Keywords: hemoglobin ; polyacrylamide electrophoresis ; crustacean ; Artemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electrophoretic mobilities of three hemoglobins (Hb1, Hb2, and Hb3) were studied in 15 populations of brine shrimps. Genetic segregation data support the model that Hb2 contains n α-polypeptides and n β-polypeptides; Hb1 contains 2n α-polypeptides. Hb3 contains neither α- nor β-polypeptides. There is no evidence of linkage of α and β loci with each other or with the locus (or loci) which governs Hb3 or with the nonhomologous portion of the sex chromosomes. Hemoglobins of different populations may be hybridized in vitro by incubation at high temperature. Reversible dissociation to subunits which contain only one (α or β) polypeptide occurs at 40 C (for Hb1) and at 50 C (for Hb2).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520188
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on the ovotestis of the slug Agriolimax reticulatus (Müller) (1976)
    Springer
    Cell & tissue research 173 (1976), S. 465-482 
    Details
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Cytochemistry ; Oocytes ; Molluscs ; Vitellogenesis ; Nucleoli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ovarian oocytes of Agriolimax reticulatus (Müller) have been studied by light and electron microscopy and electron cytochemistry. The development of the oocyte in the ovotestis may be divided into three stages. During Stage I the oocyte cytoplasm contains mainly ribosomes and also strands of endoplasmic reticulum, scattered mitochondria and Golgi systems. The nucleus contains both a paranucleolus and an eunucleolus. By Stage II the oocyte has enlarged, especially in a plane parallel to the basement membrane. In addition to the above mentioned organelles, the cytoplasm contains lipid, glycogen and early yolk platelets. During Stage III, the oocyte continues to enlarge, but mainly in a plane perpendicular to the basement membrane. A considerable degree of cytoplasmic differentiation has also taken place. The plasma membrane of the oocyte has become specialized with the appearance of a polysaccharide-rich glycocalyx, microvilli and pinocytotic tubules. Elsewhere, much of the background cytoplasm, containing Golgi-derived, polysaccharide and acid phosphatase-rich multivesiculate bodies, lipid and glycogen, is sequestered by smooth membranes and ultimately fuses with the growing yolk platelets. The nucleus contains an amphinucleolus, characteristic of many gastropods. The findings of this study are discussed in relation to results from other studies on oogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224309
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  • 4
    Electronic Resource
    Electronic Resource
    The effects of starvation on the planarian worm Polycelis tenuis iijima (1976)
    Springer
    Cell & tissue research 169 (1976), S. 193-209 
    Details
    ISSN: 1432-0878
    Keywords: Planaria ; Starvation ; Fine structure ; Biochemistry ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Employing a combination of microscopical, biochemical and autoradiographic techniques, the primary effects of starvation on adult Polycelis tenuis have been studied. Over a five week period of starvation there is on average a 32% decrease in the size of the organism. This decrease is contributed to by a reduction in mitosis and an increase in cell shrinkage autolysis and death. During starvation (following a sharp rise in RNA synthesis) there is a distinct sequence of events; four peaks of acid phosphatase activity can be resolved. The first is associated with the immediate response of the gastrodermis to feeding; the second (after 6 to 7 days) with increased autophagy and dedifferentiation in the gland cells and with muscle lysis; the third peak (after 14 to 15 days) is contributed to largely by the lysis of cells in the gut and the fourth peak (after 25 to 26 days) is caused by an extensive lysis of the reproductive system. Fine structural changes involving increased intracellular vacuolation, autophagy, crinophagy, atrophy of muscle, increased intercellular space and loss of basement membrane matrix have been related to changes in enzyme pattern. Nerve cells appear unchanged throughout the first five weeks of starvation. Pigment and gland cells loose their characteristic granules, dedifferentiate and become morphologically similar to the undifferentiated neoblasts. Dedifferentiation and the mechanisms involved in the survival of starvation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214208
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