ISSN:
0935-6304
Keywords:
Chromatography
;
Theory
;
Chromatographic systems analysis
;
Real plate number
;
Fundamental interpretation
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The real plate number concept is interpreted in terms of the fundamental properties of a chromatograph. It is shown that the linear relationship between the total peak width at half height, (b0.5)T, t, and k is only an approximation of the more general linear relationship between (b0.5)2T, t and (1 + k)2. In the case where the relationship between (b0.5)T, t and k approaches linearity, it is concluded, firstly, that b0 = a, i. e., b0 is a column property and independent of extra-column phenomena and, secondly, that nreal=ntheor. When the relationship becomes non-linear, b0 is determined partly by extra-column phenomena, but not in terms of a useful relationship. It is concluded that it is unnecessary to introduce the parameters a, b0 and nreal, since they all have counterparts in the established theory of chromatography. These counterparts prove to be equally useful in the practical analysis of chromatographic systems.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240010109
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