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  • Cell & Developmental Biology  (237)
  • Project report/description
  • 1975-1979  (131)
  • 1950-1954  (51)
  • 1935-1939  (62)
  • 1
    Electronic Resource
    Electronic Resource
    The selection of a stable rat hepatoma variant with concomitant increase in ploidy and permeability to glycerol (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 94 (1978), S. 197-203 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: By repeated selection for longer survival in an isotonic solution of glycerol, a stable subline of Novikoff rat hepatoma cells has been isolated. The cells exhibit markedly increased resistances to osmotic lysis in isotonic solutions of glycerl. They are twice as large and have twice as many chromosomes as cells of the parental line. It is suggested that the osmotic stress procedure can be extended for the selection of numerous kinds of mutants and can be used as a method of analysis of membrane properties.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040940209
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    Paper (German National Licenses)
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  • 2
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    Report of the Interdivisional Committee on Borderland Fields Between Geology, Physics, and Chemistry, 1937 (1938)
    Nat. Research Council, Div. Geology and Geography
    In:  London, Nat. Research Council, Div. Geology and Geography, vol. 159, no. 22, pp. 662-664, (ISBN 0-470-87000-1 (HB), ISBN 0-470-87001-X (PB))
    Details
    Publication Date: 1938
    Keywords: Project report/description ; Geol. aspects
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    BIBLIOGRAPHY SEISMOLOGY
  • 3
    Electronic Resource
    Electronic Resource
    A study of the fine structure of the accessory muscle of the horseshoe crab, Tachypleus gigas (1975)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 147 (1975), S. 439-457 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The accessory muscle of the walking leg of the horseshoe crab, Tachypleus gigas, was examined electron microscopically. The muscle fibers vary in size but are small in diameter, when compared with other arthropod skeletal muscles. They are striated with A, I, Z and poorly defined H bands. The sarcomere length ranges from 3-10 μm with most sarcomeres in the range of about 6 μm. The myofilaments are arranged in lamellae in larger fibers and less well organized in the smaller ones. Each thick filament is surrounded by 9-12 thin filaments which overlap. The SR is sparse but well organized to form a fenestrated collar around the fibrils. Individual SR tubules are also seen among the myofibrils. Long transverse tubules extend inward from the sarcolemma to form dyads or triads with the SR at the A-I junction. Both dyads and triads coexist in a single muscle fiber, a feature believed to have evolutionary significance. The neuromuscular relationship is unique. In the region of synaptic contact, the sarcolemma is usually elevated to form a large club-shaped structure containing no myofilaments and few other organelles. The axons or axon terminals and glial elements penetrate deep into the club-shaped sarcoplasm and form synapses with the fiber. As many as 13 terminals have been observed within a single section. Synaptic vesicles of two types are found in the axon terminals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051470405
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  • 4
    Electronic Resource
    Electronic Resource
    ‘The Linea masculina,’ a new secondary sex character in Salientia (1935)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 57 (1935), S. 131-145 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A hitherto unknown secondary sex character in male frogs consists of a series of connective tissue bands which extends the entire length of both layers of the Obliquus muscle, at both their dorsal and ventral borders. Extensions of the ventral bands may be carried inward toward the midline at certain of the inscriptiones tendinae. These bands are named the Lineae masculinae. They are clearly visible in specimens from which the skin has been removed, and are visible even through the skin in living specimens of certain species of Kaloula.Ninety-one genera and 553 species of the frogs and toads of the world were examined for the presence or absence of this structure. It is distinct in the common American and European ranids, such as Rana pipiens and Rana esculenta. It is entirely absent in many genera. When present it is found only in adult males, being absent in sexually immature males and females of all ages. Examination revealed no seasonal variations in males of Rana pipiens. This indicates that there is some correlation between the expression of this character and the sexual hormones. No function is assigned to the Linea masculina, although it is suggested that it may be associated in some way with voice production. A discussion of the taxonomic distribution of the character is included.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050570108
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Mammalian chromosomes in vitro. III. On somatic aneuploidyAided by a grant from the American Cancer Society (CP-12E). (1953)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 93 (1953), S. 301-329 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050930204
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  • 6
    Electronic Resource
    Electronic Resource
    Control of the Balb/c-3T3 cell cycle by nutrients and serum factors: Analysis using platelet-derived growth factor and platelet-poor plasma (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 99 (1979), S. 395-405 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Much controversy regarding the relationship between nutrients and serum in regulation of cell growth can be reconciled by recognizing that serum contains multiple factors which regulate different events in the cell cycle. Serum was fractioned into a platelet-derived growth factor (PDGF), which induces cells to become competent to synthesize DNA, and plasma which allows competent cells to traverse G0/G1 and enter the S phase. Nutrients are not required for the cellular response to PDGF; however amino acids are required for plasma to promote the entry of PDGF-treated, competent cells into S phase. The nutrient independent, PDGF-modulated, growth regulatory event (competence) is located 12 hours prior to the G1/S phase boundary in quiescent, density-arrested Balb/c-3T3 cells. The nutrient dependent, plasma-modulated event is located six hours prior to the G1/S phase boundary and corresponds in time to a plasma dependent growth arrest point. Moreover, plasma controls the concentration of amino acids required for DNA synthesis. Infection of density-arrested Balb/c-3T3 cells with SV40 overrides both the nutrient independent and the nutrient dependent growth regulatory events.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040990314
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  • 7
    Electronic Resource
    Electronic Resource
    Tunicamycin-mediated depletion of insulin receptors in 3T3-L1 adipocytes (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 99 (1979), S. 37-42 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Tunicamycin, an antibiotic that inhibits protein glycosylation, elicited a rapid depletion of insulin binding activity at the surface of 3T3-L1 adipocytes. Disappearance of insulin receptors occurred more rapidly in the presence of tunicamycin than when protein synthesis was inhibited by cycloheximide and was accompanied by a diminution in sensitivity of the adipocytes to the acute effects of insulin and anit-insulin receptor antibody on hexose uptake and metabolism.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040990106
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  • 8
    Electronic Resource
    Electronic Resource
    Electron probe microanalysis of chemical elemental content of single human red cellsPresented in part: Proceedings of the Microbeam Analysis Society Ninth Annual Conference, Ottawa, Canada, 1974. (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 90 (1977), S. 117-126 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Sodium, potassium, iron and sulfur contents of single human red cells were measured using electron microprobe microanalysis. Three preparative procedures were compared, and the most reliable technique was found to be spraying of cells onto polished pyrolytic graphite by atomization. Primary standards were prepared by adjusting the intracellular electrolyte content of red cells, eliminating the need to correct for X-ray absorption. Samples were stable under the electron beam during analysis, and could be stored for long periods of time. Strong correlations were found between the X-ray intensities of iron and sulfur and between potassium and sodium. X-ray intensities of potassium and sodium were found to be directly proportional to internal ionic content. Large populations of single cells could be analyzed and the distribution of their elemental content studied.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040900114
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  • 9
    Electronic Resource
    Electronic Resource
    Infrequent chromosome aberrations in syrian hamster cells following partial synchrony by amino-acid deprivation (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 86 (1975), S. 599-604 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Incubation of Syrian hamster embryo secondary cell cultures in isoleucine or arginine deficient medium inhibited cell multiplication; 4-7% of the cells were synthesizing DNA compared to approximately 40% for cells in complete medium. After the deficient medium was replaced with complete medium, cells resumed multiplication and within 12 hours 65-70% of the cells entered into the S phase. A second peak of labeled cells, approximately 55%, occurred 24 hours after addition of complete medium. Chromosomal damage characteristic for cell lines grown in amino acid deficient medium did not occur with these Syrian hamster cells. Isoleucine or arginine deprivation resulted in chromosome or chromatid aberrations in only 2-5% of the cells, whereas chromosomes of control cells in complete medium occasionally exhibit chromatid gaps (〉 1% of the cells).
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040860505
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  • 10
    Electronic Resource
    Electronic Resource
    Differences in the incorporation of thymidine into DNA of normal and cystic fibrosis fibroblasts in vitroGrant support DHEW 5P01 AM 17040(02) (Program Projects B and F). (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 88 (1976), S. 33-41 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Although similar fractions of cells were in the S phase of the cell cycle, normal human skin fibroblasts were shown to incorporate more than twice the 3HTdR into their DNA in vitro than did cells obtained from individuals with cystic fibrosis (CF). Obligate heterozygotes incorporated an intermediate amount of the DNA precursor. Studies were initiated to determine the basis of the differential incorporation of 3HTdR among the genotypes. An analog of thymidine, BUdR, produced varied effects on the growth kinetics of the three genotypes. The growth of cells in BUdR resulted in a 50% increase in the population doubling times of all three genotypes, and caused the cell morphology to change from a spindle shape to one in which the cells became broadened and flat, with numerous cytoplasmic projections extending for distances of several cell diameters. The activities of thymidine kinase and the participation of the exogenous and de novo pathways in the synthesis of TMP were found to be approximately the same in all three genotypes. The data suggest that an alteration in the transport of thymidine into the cells may account for the differences in TdR incorporation into DNA, and this may be associated with other changes in cystic fibrosis that are apparently membrane associated.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040880105
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