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  • 1
    Electronic Resource
    Electronic Resource
    The cardiac muscle in the pulmonary vein of the rat: A morphological and electrophysiological studyWork done in the Department of Morphology of the Faculty of Medicine of Ribeirão Preto, University of São Paulo, and Institute of Biophysics of the Federal University of Rio de Janeiro. Supported in part by the Fundação de Amparo à Pesquisa do Estado de São Paulo, grant 71/490 - médicas; by the Conselho de Pesquisas da Universidade Federal do Rio de Janeiro, by the Conselho Nacional de Pesquisas (Brasil), and by Banco Nacional de Desenvolvimento Econǒmico (Brasil) Contrato FUNTEC 143. (1975)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 145 (1975), S. 409-433 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The pulmonary veins of albino Wistar rats were studied by means of light and electron microscopy. The media of larger veins consists of cardiac muscle fibers which extend until the vessels attain about 100 μ in diameter. This coat consists of external longitudinal fibers and internal circular fibers. The vasa vasorum are well developed and the capillaries show pseudofenestrations. The numerous adrenergic and cholinergic nerve endings do not form typical motor end-plates as seen in skeletal muscles. The ultrastructure of these media muscle fibers is similar to that of rat hearts. The smooth muscle layer of larger pulmonary veins is not continuous as it is in smaller veins where it forms cushions. Comparisons of albino rats and other rodents reveal striking differences.Action potential shape and propagation velocity (0.5-1.2 m/s) along the myocardial coat of the pulmonary vein were similar to those observed in the left atrium and so was their sensitivity to locally applied acetylcholine. The physiological direction of propagation in rat pulmonary veins is toward the lung. This finding lends support to the hypothesis of a rhythmic, valve-like action of the striated musculature of the pulmonary venous wall during the systole and a possible role in the capacitance of the pulmonary circulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051450403
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  • 2
    Electronic Resource
    Electronic Resource
    The fate mapping of the eleventh and twelfth day mouse otocyst: An in vitro study of the sites of origin of the embryonic inner ear sensory structures (1978)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 157 (1978), S. 249-267 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An experiment was undertaken to determine which sensory structures of the mouse embryo inner ear developed from what portion of the mouse otocyst. Otocysts of gestation days 10, 11, 12 and 13 were divided by surgical dissection into six anatomical groups: dorsal, ventral, anterior, posterior, medial and lateral halves. They were organ cultured separately. After a period of ten days, the explanted tissues were harvested and processed histologically for microscopic analysis. The surgical control specimens fixed at the time of explanation were composed of undifferentiated ectodermal cells for tissues of gestation days 10, 11, and 12. Otocysts of gestation day ten showed no gross morphological differentiation. Otocysts of gestation days 11 and 12 showed, during the course of their subsequent growth, that the three semicircular ducts and their associated cristae developed from the dorsal and lateral halves. Only the anterior and posterior canals and cristae originated from the medial portion. The posterior half gave rise to the posterior crista and the anterior half provided for the development of the anterior and lateral cristae. The cochlear duct and its sensory epithelium developed in all the anatomical groups except the dorsal half. The utricle developed in the dorsal section of the middle third of the otocyst, while the utricular macula developed in the anterior half of the same section of the otocyst. The saccule and its macula differentiated from the ventral section of the middle third of the anterior half.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051570302
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  • 3
    Electronic Resource
    Electronic Resource
    Presence and development of a sensory formation in the eyestalk of the crayfish, Astacus leptodactylus (Nordmann, 1842), during its embryonic, larval and adult lifeThis study was partially sponsored by C.N.R.S. (France) under grànt A.T.P. International 1976-2602. (1979)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 161 (1979), S. 39-51 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A sensory papilla is described in the eyestalk of the crayfish Astacus leptodactylus during the last embryonic stages and during larval stages by light microscopy. This region was also investigated with the scanning electron microscopy, which showed sensory hairs in the postmolt adult; they disappear during intermolt and premolt. Simultaneous cyclic changes in hair papillae are observed in the hypodermis. The possibility of a chemoreceptive function is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051610104
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  • 4
    Electronic Resource
    Electronic Resource
    Anatomical observations on the ending of the human thoracic ductSupported by FundaççãTo de Amparo a Pesquisa do Estado de São Paulo, Brazil. (1977)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 153 (1977), S. 363-369 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Fifty-one cadavers of human adults of both sexes were examined for the raminfications and site of opening of the cervical segment of the thoracic duct. It was found that the duct opened into veins of the neck through either one (84.31%) or two branches (15.69%). The most frequent site of opening was in the junction of the left internal jugular vein with the subclavian vein (18 individuals), but another frequent site (15 individuals) was in the internal jugular vein. The duct opened at the angle formed by the internal jugular vein and the internal posterior jugular vein in five individuals, and at a variety of the other locations in the remaining cadavers. These results are compared with the findings of previous investigators.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051530303
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  • 5
    Electronic Resource
    Electronic Resource
    Glucose-6-Phosphate dehydrogenase activity in a hepatoma cell line: Preliminary evidence for negative genetic controlA preliminary report of this work appeared in abstract form in the proceedings of the Thirteenth Annual Meeting, The American Society for Cell Biology, Nov. 14-17, 1973. J. Cell Biol., 59: 77a. (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 87 (1976), S. 101-109 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A line of cells, positive for glucose-6-phosphate dehydrogenase activity, has been separated from a negative parental stock derived from the H4-II-E-C3 rat hepatoma line. Cytogenetic analyses showed specific marker chromosomes of the parent line were present in the variant. On the other hand, cells of the variant line contained three chromosomes less, on the average, than the total present in parent cells. Karyotypic analyses indicated the diminution in chromosome numbers to be in the subacrocentric group. The appearance of glucose-6-phosphate dehydrogenase associated with loss of chromosomes suggests this enzyme is suppressed or under negative control in the parental stock. The consistent loss of chromosomes of the subacrocentric group suggests that the locus bearing regulatory activity is in this specific group.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040870113
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  • 6
    Electronic Resource
    Electronic Resource
    The role of cyclic AMP in myogenesis (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 86 (1975), S. 83-90 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effect of exogenously administered cyclic AMP derivatives and of endogenously elevated cyclic AMP levels on the spontaneous fusion of skeletal muscle myoblasts has been investigated. Contrary to earlier reports, cAMP does not appear to have a direct inhibitory effect on the fusion of an established line (L8) of rat myoblasts. Similarly, cAMP did not block the fusion of primary chick myoblasts. However, fusion of the rat myoblasts was prevented when the cAMP induced inhibition of growth prevented the cells from reaching the “critical” cell density necessary for fusion.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040860110
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  • 7
    Electronic Resource
    Electronic Resource
    Relationship of energy production to gluconeogenesis in renal cortical tubulesA preliminary portion of this work was presented at the meeting of the Federation of American Societies for Experimental Biology, Atlantic City, New Jersey, April 8-12, 1974. (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 86 (1975), S. 111-119 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Isolated tubules prepared by collagenase treatment of rat renal cortex retained their ultrastructural integrity and responded to added lactate and succinate with an increase in gluconeogenesis and respiration. Inhibition of the mitochondrial respiratory chain with rotenone, or energy conservation sites with oligomycin caused a marked reduction in respiration and ATP content thereby completely inhibiting net gluconeogenesis. Dissociation of gluconeogenesis from respiration was accomplished with quinolinic acid and hydrazine, inhibitors of gluconeogenesis. At 5 × 10-3 M quinolinic acid, gluconeogenesis from succinate was inhibited approximately 50% and from lactate nearly 100%. This concentration of quinolinic acid did not affect oxygen uptake or the ATP content of tubules in the presence or absence of substrate. Hydrazine at 10-3 M resulted in approximately 75% inhibition of glucose formation from succinate and complete inhibition from lactate without interfering with respiration or ATP content. The increased mitochondrial energy generation, as manifested by accelerated respiration was independent of gluconeogenesis. The unchanging cell ATP concentration with a higher respiratory rate upon addition of exogenous substrate bespeaks increased ATP turnover. ATP utilization for the substrate-induced enhancement of gluconeogenesis could not account for the increment in ATP hydrolysis.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040860113
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  • 8
    Electronic Resource
    Electronic Resource
    Dissociation by cytochalasin B of movement, DNA synthesis and transport in 3T3 cells (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 85 (1975), S. 579-585 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cytochalasin B was used as a tool to study the inter-relationships between cell movement, the reinitiated DNA synthesis and the enhanced transport of specific small molecules stimulated by serum in quiescent 3T3 cells. Cytochalasin at concentrations of less than 1 μg/ml inhibits serum-stimulated movement within the monolayer and migration into a wound. Even at ten times this concentration there is little effect on the increase in DNA in the culture, indicating that movement away from neighboring cells is not required for the initiation of DNA synthesis.While DNA synthesis is not inhibited by concentrations of cytochalasin up to 10 μg/ml, the increased thymidine transport which is associated with the onset of the S phase of the cell cycle is inhibited and DNA synthesis cannot be measured by the labelling of nuclei with radioactive thymidine.Cytochalasin has a differential effect on the early transport changes produced by serum addition. Glucose transport is inhibited by low concentrations of the drug (〈 1 μg/ml) while the enhanced uptake of phosphate and uridine is unaffected by a 10-fold increase in concentration. Although the doses of cytochalasin required for 50% inhibition of hexose uptake and of cell movement are the same, no causal relationship between sugar transport and locomotion can be demonstrated.Cytochalasin affects membrane functions in at least two different ways. The drug inhibits the uptake of glucose directly but affects only the S-phase associated increase in thymidine transport.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040850309
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of hydrocortisone on cell morphology in C6 cells (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 94 (1978), S. 321-333 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Hydrocortisone has been found to induce cell spreading in rat glial C6 cells by 24 hours after its addition. This spreading phenomenon is correlated with an increase in the fraction of the peripheral cytoplasm occupied by microfilaments. Cytochalasin B causes disorganization of microfilaments in the peripheral cytoplasm of the cells. Additionally, it also prevents cell spreading in response to hormonal stimulation. High levels of calcium prevent recovery of normal microfilament organization and cell spreading following removal of cytochalasin B, but have no effect on normal microfilament organization alone. Additionally both the hydrocortisone induced spreading of C6 cells and increases in peripheral microfilaments are shown to be dependent on RNA and protein synthesis. The levels of protein co-electrophoresing with actin are not effected by hydrocortisone.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040940310
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  • 10
    Electronic Resource
    Electronic Resource
    Diffusible factor(s) controlling density inhibition of 3T3 cell growth: A new approach (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 96 (1978), S. 327-332 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In 3T3 Swiss mouse fibroblasts, incorporation of phosphate into cells and phosphorylation of small organic compounds were increased by shaking dense cultures. This response was not obtained with SV40 transformed Swiss 3T3 cells (SV-3T3).It appeared likely that these results could be accounted for by an inhibitor released from 3T3 cells but not from SV-3T3 cells.Our new method of co-incubation of sparse and dense cultures allowed us to demonstrate inhibition of growth and phosphate metabolism in sparse 3T3 cultures which were shaken in the presence of dense cultures. The inhibition was much less when the cultures were co-cultivated but not shaken.The inhibition of phosphate incorporation in acid-soluble and acid-insoluble fractions of sparse cultures was observed as early as 20 minutes of co-incubation in the presence of dense cultures, so this inhibition is not the result of depletion of growth factors in the medium.Our experiments suggest that an inhibitor(s) was released from dense cultures of 3T3 cells.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040960308
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