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  • Chemistry  (127)
  • Electron microscopy  (5)
  • Monocytes  (4)
  • Magnetism
  • Nederland
  • 1975-1979  (52)
  • 1970-1974  (83)
  • 1
    Electronic Resource
    Electronic Resource
    Reconstitution of the thymus dependent area in the spleen of lethally irradiated mice (1974)
    Springer
    Cell & tissue research 149 (1974), S. 43-60 
    Details
    ISSN: 1432-0878
    Keywords: Spleen ; Thymus dependent area ; Periarteriolar lymphatic sheath (PALS) ; Interdigitating cell (IDC) ; T-lymphocyte ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To study the submicroscopical morphology of the microenvironment for T-lymphocytes in the spleen, mice were lethally X-irradiated and injected intravenously with syngeneic thymocytes. 24 hours after cell transfer, small lymphocytes occurred in the thymus dependent area of the spleen: the periarteriolar lymphatic sheath (PALS). They localized preferentially around a special type of mononuclear phagocyte, the Interdigitating Cell (IDC), which is considered to be characteristic for thymus-dependent areas in peripheral lymphoid organs. A close cell contact between both cell types was observed: small lymphocytes protruded into the cytoplasm of the IDC by means of fingerlike protrusions. This type of cell contact seems to induce blast transformation of the lymphoid cells which resulted in the formation of medium sized T-cells. In a control experiment, spleen cells from thymectomized, X-irradiated and bone marrow reconstituted mice were injected intravenously into lethally X-irradiated recipients. These B-lymphocytes, however, were not found to be localized around IDC. They preferentially formed primary follicles at the periphery of lymphocyte-depleted thymus dependent areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209049
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  • 2
    Electronic Resource
    Electronic Resource
    Peroxidatic activity in monocytes and tissue macrophages of mice (1978)
    Springer
    Cell & tissue research 188 (1978), S. 361-373 
    Details
    ISSN: 1432-0878
    Keywords: Macrophages ; Monocytes ; Peroxidatic activity ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A description is given of the distribution of peroxidatic (PO) activity in murine monocytes of blood and peritoneal cavity, and in murine macrophages residing in the unstimulated peritoneal cavity as well as in liver, spleen, bone marrow, and small intestine. In the monocytes, PO activity is restricted to some of the cytoplasmic granules; in the tissue (or resident) macrophages present in peritoneal cavity, liver, spleen, and small intestine, the PO activity is located in the nuclear envelope and the rough endoplasmic reticulum. Macrophages in the bone marrow are PO-negative. In the spleen and bone marrow, reticulum cells show PO activity in the nuclear envelope and the RER. Transitional forms between monocytes and tissue macrophages were not observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219778
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  • 3
    Electronic Resource
    Electronic Resource
    Determination of the base-pairing content of four specific tRNAs by infrared spectroscopy (1977)
    New York : Wiley-Blackwell
    Biopolymers 16 (1977), S. 1735-1745 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Infrared spectroscopy was used for the determination of the base-pairing content of four specific tRNAs in deuterium oxide solution. Infrared spectra were obtained in the 1750-1550 cm-1 region at various temperatures ranging from about 15 to 90°C. Melting curves were constructed by plotting the molar extinction coefficient at ν = 1657 cm-1 versus temperature. These transition curves enabled us to determine the ranges of temperature which correspond to the ordered (partially double-stranded) or randomly coiled structure of the tRNA. For a set of wavenumbers the extinction coefficients at these temperatures were used for the calculation of the base-pairing content. The procedure employed here is based on a method described earlier by Thomas [(1969) Biopolymers 7, 325-334]. For the conditions selected for this investigation (Mg2+-free D2O-buffer; 0.01M tris-DCl, 0.015M NaCl, pD 7.5) the results of this determination agree within the limits of errors with the number of base pairs predicted by the cloverleaf model.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1977.360160810
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  • 4
    Electronic Resource
    Electronic Resource
    An electron microscopic method for studying nucleic acid-protein complexes. Visualization of RNA polymerase bound to the DNA of bacteriophages T7 and T3Dedicated to the memory of Dr. Jean-Pierre Tranzer. (1974)
    New York : Wiley-Blackwell
    Biopolymers 13 (1974), S. 995-1009 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Double-stranded DNA can be readily adsorbed on mica or directly on carbon coated grids from the surface of solutions containing ethidium bromide, actinomine, or propidium diiodide. The DNA molecules are unfolded, well separated, and show a length distribution similar to molecules prepared by protein monolayer techniques.Since the intercalating dyes tested do not lead to an increased apparent diameter of the nucleic acid the method is useful for the study of nucleic acid-protein complexes. As a model, the binding of E. coli RNA polymerase to phage T7 and T3 DNA was examined under different conditions. The enzyme can easily be identified and its position along the DNA molecule can be mapped.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1974.360130514
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  • 5
    Electronic Resource
    Electronic Resource
    Zellphysiologie des Stoffwechsels. Von D. Pette. Bd. 13 der Konstanzer Universitätsreden. Herausgeg. von G. Hess. Konstanzer Universitätsverlag 1970. 50 S., brosch. DM 4,80 (1970)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 4 (1970), S. 196-196 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19700040613
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  • 6
    Electronic Resource
    Electronic Resource
    Physikalische Methoden in der Chemie: UV-Photoelektronenspektroskopie (1971)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 5 (1971), S. 65-77 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 19 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19710050302
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  • 7
    Electronic Resource
    Electronic Resource
    Notiz über ein einfaches Verfahren zur Darstellung von 3,4-Bismethylencyclobuten (1973)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 106 (1973), S. 3461-3462 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19731061031
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  • 8
    Electronic Resource
    Electronic Resource
    [2.2]Paracyclophane durch Addition von Acetylenderivaten an 1,2,4,5-Hexatetraen (1974)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 107 (1974), S. 1891-1902 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Preparation of [2.2]Paracyclophanes by Addition of Acetylene Derivatives to 1,2,4,5-HexatetraeneThe tetrasubstituted [2.2]paracyclophanes 4a - e are formed by cycloaddition of the symmetrical acetylene derivatives 2a - e to 1,2,4,5-hexatetraene (1), and their constitution and configuration is established by spectroscopic methods, degradation and pyrolysis experiments. The tetramethyl ester 4a is reduced to the tetraalcohol 4g, and the tetracarboxylic acid 4c is converted to the bisanhydride 4f and [2.2] paracyclophanes (4h), respectively. All possible disubstituted [2.2] paracyclophanes 6 are obtained when methyl propiolate (5a) is added to. 1, whereas further symmetrical and unsymmetrical acetylene derivatives do not react with 1.
    Notes: Die tetrasubstituierten [2.2]Paracyclophane 4a - e werden durch Cycloaddition der symmetrischen Acetylenderivate 2a - e an 1,2,4,5-Hexatetraen (1) dargestellt und ihre Konstitution und Konfiguration durch spektroskopische Methoden. Abbau- und Pyrolyseversuche bewiesen. Der Tetramethylester 4a wird zum Tetraalkohol 4g reduziert und die Tetracarbonsäure 4c in das Bisanhydrid 4f bzw. [2.2] Paracyclophan (4h) übergeführt. Bei der Anlagerung von Propiolsäure-methylester (5a) an 1 werden alle isomeren disubstituierten [2.2]paracyclophanes 6 erhalten. Weitere symmetrische und unsymmetrische Acetylenderivate reagieren nicht mit 1.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19741070613
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  • 9
    Electronic Resource
    Electronic Resource
    Free radicals in colloidal systems: Formation and decay of radicals in the tensides α-hexadecyl-ω-hydroxypoly(oxyethylene) and α-tetradecyl-ω-(sodium sulfonato)tri(oxyethylene) (1978)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 179 (1978), S. 2279-2295 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Organic radicals were produced in the tensides C16H33(OCH2CH2)21OH and C14H29(OCH2CH2)3-SO3Na in aqueous solutions using a short pulse of high energy electrons. The radicals were formed by OH attack on the (OCH2CH2)x-parts of the tensides. The decay of the 250nm absorption of the radicals was recorded at different initial radical concentrations and tenside concentrations. Several radicals could be produced in one micelle. Radicals formed in the same micelle decay within microseconds or faster. The half life time τ1 in a micelle carrying two radicals is 2,0·10-6 s for C16H33(OCH2CH2)21 OH and less than 6·10-7 s for C14H29(OCH2CH2)3SO3Na. A model for intramicellar radical-radical reactions is proposed according to which the rate is faster in tensides of high critical micelle concentration. Single radicals in micelles of C16H33(OCH2CH2)21OH can deactivate each other without leaving the micelles. This intermicellar reaction is discussed in terms of the rate of diffusion-controlled micelle-micelle encounters, an encounter time of 7·10-8s, and the above time τ1 for intramicellar reaction. The observed rate constant 2k of intermicellar reaction is 3,5·106 mol-1·l·s-1. At low tenside concentrations, the bimolecular rate constant increases since more single tenside radicals are present in solution. They react rapidly (ca. 108 mol-1·l·s-1) with radicals in micelles. Single radicals in C14H33(OCH2CH2)3-SO3Na micelles cannot directly react with each other because of the Coulombic repulsion between two anionic micelles. Reaction occurs after the exit of a tenside radical from its micelle, the rate of which depends on the micellar equilibrium Mn⇄Mn-1+M (M: tenside molecule; n: agglomeration number of micelle). A single radical in solution reacts with a single radical in a micelle with 2k = 1,0·108 mol-1·l·s-1 and with another single radical in solution with 4,0·107 mol-1·l·s-1.γ-Irradiation of both tensides in aqueous solution leads to slight increases in viscosity, followed by turbidity beyond the “gel dose” and phase separation. These effects are explained in terms of crosslinking of tenside molecules. Formation of a large network requires bridges between all participating tenside molecules (not only bridges between micelles).
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1978.021790915
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  • 10
    Electronic Resource
    Electronic Resource
    Wärmealterung und Grenztemperatur von organischen Isolierstoffen (1971)
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 2 (1971), S. 127-135 
    Details
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Thermal aging and endurance of organic insulating materials. The knowledge of the thermal endurance of organic insulating materials is very important for the application in electrical engineering. The change of the values of the properties by thermal aging is, theoretically, described under the assumption being a linear connection to the rules of the kinetic theory of chemical reactions. By this premise it is possible to indicate functions for aging and thermal endurance, being plotted as straight lines in suitable coordinates. In this way the test results can be evaluated better than by conventional means.
    Notes: Die Kenntnis der Grenztemperatur organischer Isolierstoffe ist für deren Anwendung in der Elektrotechnik von großer Wichtigkeit. Die Änderung von Eigenschaftswerten auf Grund einer Wärmebehandlung wird theoretisch beschrieben unter der Annahme, daß ein linearer Zusammenhang mit den Gesetzen der Reaktionskinetik besteht. Unter den getroffenen Voraussetzungen können. Alterungs- und Lebensdauerfunktionen angegeben werden, die sich bei geeigenten Koordinaten als Geradendarstellen lassen. Meßergebnisse können auf diese Weise besser ausgewertet werden als nach herkömmlichen Verfahren.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mawe.19710020304
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