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  • Articles  (120)
  • Physical Chemistry  (67)
  • Life Sciences  (40)
  • Gas chromatography
  • Wiley-Blackwell  (120)
  • 1975-1979  (85)
  • 1970-1974  (35)
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  • Articles  (120)
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 8 (1976), S. 23-24 
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 11 (1979), S. 175-185 
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Relaxation rates for O2(1Σg+) by nonradiative pathways have been determined using the fast-flow technique. O2(1Σg+) is formed from O2(1Δg) by an energy pooling process. O2(1Δg) is generated by passing purified oxygen through a microwave discharge. Oxygen atoms are removed by distilling mercury vapor through the discharge zone. It has been observed that the wall loss rate for O2(1Σg+) decreases with increasing pressure of oxygen and thus appears to be diffusion controlled. Quenching rate constants for O2, N2, and He have been determined and found to be (1.5 ± 0.1) × 104, (1.0 ± 0.05) × 106 and (1.2 ± 0.1) × 105 l./mol·sec, respectively.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 2 (1974), S. 558-581 
    ISSN: 0091-7419
    Keywords: Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Internal dialysis techniques have been used to examine the influence of external and internal cations on Ca efflux from ATP-depleted squid axons. The main observation is that Ca efflux is promoted by external Na and inhibited by internal Na. The Na0 -dependent Ca efflux appears to be a function of [Na]03, and is also affected by the membrane potential; a 25 mV depolarization may cause as much as an e-fold decrease in Ca efflux. These data are consistent with a counter-transport exchange of 3Na+-for-1Ca2+. A Ca0-dependent Ca efflux has also been observed; it is prominent in Na sea water or Le sea water, and is markedly diminished in choline sea water. This flux is consistent with the idea of a Ca-Ca exchange diffusion process. Taken together, the Na0 - and the Ca0 -dependent Ca effluxes fit a two-site model for carrier-mediated Ca transport; one site binds two Na+ or one Ca2+, while the second site can bind either one Na+ or one Li+. The data reported here suggest that both sites must be filled on the inward journey, but that only the Ca-binding site need be occupied on the outward journey of the carrier. A mechanism of this type could derive sufficient energy from the Na and voltage gradients to maintain a [Ca2+]0/[Ca2+]i concentration ratio of about 104 in the absence of ATP. The present experiments do not, however, rule out the possible participation of a metabolically driven Ca transport mechanism in vivo.
    Additional Material: 14 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 6 (1977), S. 363-374 
    ISSN: 0091-7419
    Keywords: thymidine transport ; nitrobenzylthioinosine ; bromodeoxyuridine resistances ; HeLa cells ; thymidine kinase ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A line of HeLa cells resistant to 5-bromo-2′-deoxyuridine (BUdR) was established by continuous culture in growth medium containing BUdR; during the selection period, BUdR concentrations, initially 15 μM, were gradually increased to 100 μM. Cells of a clone (HeLa/B5) established from this line were also resistant to 5-fluoro-2′-deoxyuridine (FUdR), but not to the free base, 5-fluorouracil. Although extracts of HeLa/B5 cells exhibited levels of thymidine kinase activity comparable to those of parental cells, rates of uptake of BUdR, FUdR, and thymidine into intact cells were much reduced. The kinetics of uptake of uridine and adenosine, nucleosides which appear to be transported independently of thymidine in HeLa cells, were similar for HeLa/B5 and the parental line (HeLa/0). Relative to thymidine uptake by HeLa/0 cells, that by HeLa/B5 cells was distinctly less sensitive to nitrobenzlthionosine (NBMPR), a specific inhibitor of nucleoside transport in various types of animal cells. Despite this difference in NBMPR sensitivity, both cell lines possessed the same number of high affinity NBMPR binding sites per mg cell protein. The altered kinetics of thymidine uptake and the NBMPR insensitivity of that function in HeLa/B5 cells suggest that resistance to BUdR is due to an altered thymidine transport mechanism.
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  • 5
    ISSN: 0091-7419
    Keywords: cell adhesion ; adhesion proteins ; fibronectin ; chondronectin ; collagen substrates ; gangliosides ; cell surface ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Fibronectin mediates the adhesion of fibroblasts to collagen substrates, binding first to the collagen and then to the cells. We report here that the interaction of the cells with the fibronectin-collagen complex is blocked by specific gangliosides, GD1 a and GT1, and that the sugar moieties of these gangliosides contain the inhibitory activity. The gangliosides act by binding to fibronectin, suggesting that they may be the cell surface receptor for fibronectin. Evidence is presented that other adhesion proteins or mechanisms of attachment exist for chondrocytes, epidermal cells, and transformed tumorigenic cells, since adhesion of these cells is not stimulated by fibronectin. Chondrocytes adhere via a serum factor that is more temperature-sensitive and less basic than fibronectin. Unlike that of fibroblasts chondrocyte adhesion is stimulated by low levels of gangliosides. Epidermal cells adhere preferentially to type IV (basement membrane) collagen but at a much slower rate than fibroblasts or chondrocytes. This suggests that these epidermal cells synthesize their own specific adhesion factor. Metastatic cells cultured from the T241 fibrosarcoma adhere rapidly to type IV collagen in the absence of fibronectin and do not synthesize significant amounts of collagen or fibronectin. Their growth, in contrast to that of normal fibroblasts, is unaffected by a specific inhibitor of collagen synthesis. These data indicate the importance of specific collagens and adhesion proteins in the adhesion of certain cells and suggest that a reduction in the synthesis of collagen and of fibronectin is related to some of the abnormalities observed in transformed cells.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 6 (1977), S. 389-398 
    ISSN: 0091-7419
    Keywords: amino acid transport ; transport energetics ; cytochrome-deficient mutant ; shock-sensitive transport ; shock-resistant transport ; Salmonella typhimurium ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The effect of inhibitors and uncouplers on the osmotic shock-sensitive transport systems for glutamine and galactose (by the β-methyl galactoside permease) was compared to their effect on the osmotic shock-resistant proline and galactose permease systems in cytochrome-deficient cells of Salmonella typhimurium SASY28. Both osmotic shock-sensitive and -resistant systems were sensitive to uncouplers and to inhibitors of the membrane-bound Ca2+, Mg2+-activated adenosine triphosphatase. This suggests that uptake by both types of systems is energized in these cells by an electrochemical gradient of protons formed by ATP hydrolysis through the ATPase.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 6 (1974), S. 245-256 
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The kinetics of N-phenylphthalamic acid and of N-phenylphthalimide hydrolysis in aqueous solutions of sulfuric acid has been studied. A reaction mechanism is proposed implying that unreactive forms of the reactant appear by protonization of the amide bond at the carbonyl oxygen and by dissociation of the o-carboxyl group (N-phenylphthalamic acid). Attack of the nonprotonized amide bond by the hydroxonium ion is suggested to be the rate-limiting step.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 11 (1979), S. 357-374 
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Rate, equilibrium, and thermodynamic data for reaction (1) of 2,6-diphenyl-4R-phenoxyl radicals, where R==OCH3 (I), Ph (II), OC2H5 (III), O-n-C18H37 (IV), and 2,6-dicyclohexyl-4-phenylphenoxyl radical (V), in various solvents are obtained. The k1 values of radicals I to V are within (5.5 ± 1.0) × 107-(1.4 ± 0.3) × 109M-1·sec-1 in propanol. The solvent effect on k1 for radicals I and II was studied. The dimerization of radical I is diffusion-controlled in all solvent studies. The dimerization of radical II is viscosity-dependent but not diffusion-controlled. Plots of k1 against ET have a V shape. Specific solvent-solute interactions are seeming to be responsible for numerical k1 values of radicals I and II. The solvent effect is more pronounced for “slow” dimerization of radicals II than for “fast” dimerization of radicals I. The minimum k1 values correspond to pyridine and chloroform. The reaction (1) rate strongly depends upon the composition of a chloroform (S)-cosolvent binary mixture. Besides reaction (1) the following reactions proceed in binary mixture: \documentclass{article}\pagestyle{empty}\begin{document}$$ K_{14} = 0.18 \pm 0.05M^{ - 1},k_{15} = (2.0 \pm 1.0) \times 10^8 M^{ - 1} \cdot \sec ^{ - 1} $$\end{document} (radical I, S-CCL4 mixture) \documentclass{article}\pagestyle{empty}\begin{document}$$ K_{14} = 0.9 \pm 0.2M^{ - 1},k_{15} = (1.2 \pm 0.5) \times 10^7 M^{ - 1} \cdot \sec ^{ - 1} $$\end{document}(radical II, S-C6H14 mixture) \documentclass{article}\pagestyle{empty}\begin{document}$$ K_{14} = 0.45 \pm 0.10M^{ - 1},k_{15} = (9.0 \pm 2.0) \times 10^6 M^{ - 1} \cdot \sec ^{ - 1} $$\end{document}(radical II, S-CCL4 mixture)In all cases k16 ≪ k15. Factors influencing dimerization rates in strongly nonideal mixtures CH3OH-CCL4 and CH3OH-CHCl3 are discussed.
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 288-292 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Stationary phases, polar, gum type ; For temperatures up to 300°C ; For most of the common classes of organic compounds ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Most of the common classes of organic compounds chromatograph normally on Superoxes. There is no tailing or adverse effect from excessively different activity different activity coefficients. Superoxes are therefore universal phases for gas chromatography. This is also expressed by a wide useful temperature range from ∼50° to ∼300°. High MW Superox-4 has a MAOT about 20° higher than the lower MW Superox-0.1. Several applications illustrating the versatility of Superox phases in (GC)2 are presented.
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 283-287 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Sampling technique ; Semi-automation of head space sampling ; Application to water pollution, coffee aroma, light hydrocarbons in crude oil ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Head space GC using a special electropneumatic sampling system works well in combination with glass capillaries. Because a homogenous gas mixture is already present most problems inherent with inlet splitters are thus avoided. In cases where the high vapor pressure of the sample can cause problems with the pressure controlled sampling system, the use of narrow bore glass capillaries provides the necessary inlet pressure. The use of this powerful and convenient analytical tool for water pollution analysis, flavour analysis and the analysis of volatiles in crude oil samples is shown by examples.
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