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  • 1980-1984  (3)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Lytic and bactericidal properties of salmonid sera (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 23 (1983), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: There are two types of haemolytic activity against heterologous red blood cells in the sera of fishes including salmonids: a specific antibody-dependent complement-mediate activity ‘CH50’ and a natural, non-specific activity ‘SH50’. Sera of coho salmon, Oncorhynchus kisutch, masu salmon, O. masou, rainbow trout and steelhead trout, Salmo gairdneri, were assayed for CH50 and SH50 activities by incubation at 30°C for 60 min with goldfish red blood cells (GFRBC) sensitized with the rainbow trout antiGFRBC serum and unsensitized GFRBC for CH50 and SH50, respectively. SH50 activity was usually half that of CH50 in each serum. In healthy individuals, both CH50 and SH50 activities were confined to a narrow range specific for each species. While EDTA abolished both haemolytic activities, moieties of the activities remained in the presence of EGTA. Incubation with lipopolysaccharide and zymosan reduced both CH50 and SH50 activities. Fish serum possessed a natural, powerful bactericidal action, which for Aeromonas salmonicida was proportional to the level of SH50 activity. Changes in the SH50 activity were related to starvation and infectious diseases. The SH50 activity decreased as the diseases (furunculosis and vibriosis) progressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1983.tb02926.x
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  • 2
    Electronic Resource
    Electronic Resource
    The non-specific activation of rainbow trout, Salmo gairdneri Richardson, complement by Aeromonas salmonicida extracellular products and the correlation of complement activity with the inactivation of lethal toxicity products (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 7 (1984), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract The possible mechanism of inactivation of the toxicity of Aeromonas salmonicida extracellular products (ECP) by normal rainbow trout serum was investigated using juvenile rainbow trout. ECP was prepared from culture supernatant by an acetone precipitation method. The ECP was incubated with normal rainbow trout serum at 20°C for 2 h, and the interrelationship between ECP proteolytic activity and immune complex-initiating, haemolytic complement activity (CH50) of normal serum against antibody-sensitized goldfish red blood cells was evaluated. When normal serum was incubated with increasing concentrations of ECP, the CH50 activity of serum decreased. The CH50 activity was completely abolished in serum treated with undiluted ECP. ECP treated with serum was administered to trout intraperitoneally to determine mortality. All the fish receiving untreated ECP (0.05 ml = 0.5 mg protein) alone died within 24 h. When ECP was treated with serum at 1:1 to 4:1 (serum: ECP) in volume a similar high mortality was produced. These inocula possessed high protease activity and no or low CH50 activity. However, mortality decreased and finally no mortality was recorded as ECP was treated with large volumes of serum (9:1 to 19:1). These inocula had lower protease activity and considerably higher CH50 activity. Fish receiving ECP treated with heat-inactivated serum at 19:1 showed 100% mortality. A serum: ECP inoculum derived from fish which had been administered lipopolysaccharide from Salmonella enteritidis and which possessed a low CH50 activity also gave a high mortality when used at 19:1. These results suggest that rainbow trout complement is implicated in the inactivation of toxicity of A. salmonicida ECP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1984.tb01198.x
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  • 3
    Electronic Resource
    Electronic Resource
    Opsonization by fish antibody and complement in the immune phagocytosis by peritoneal exudate cells isolated from salmonid fishes (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 7 (1984), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Opsonic activities of fish antibody and complement in the phagocytic responses of fish leucocytes have been investigated using salmonid peritoneal exudate cells (PEC) and viable cells of Aeromonas salmonicida as antigenic particles. PEC were isolated from liquid paraffin-injected coho salmon, masu salmon and rainbow trout. After incubation at 20°C for 2 h, phagocytosis by PEC was evaluated. Phagocytic activity was attributed to neutrophil-like and macrophage-like cells, though in the absence of immune serum activity was low. The addition of fish antibody (heal-inactivated antiserum) slightly increased the activity. On the addition of normal serum as a complement source phagocytic activity was dramatically accelerated. This acceleration was interpreted as opsonization as a result of the classical pathway activation of fish complement by an antigen-antibody reaction. The complement of mammals (goat, guinea pig and rabbit) did not possess opsonic activity in the presence of fish antibody.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1984.tb00904.x
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