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  • 1980-1984  (8)
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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of mitochondrial DNA of the oomycetous fungus Phytophthora infestans (1984)
    Springer
    Current genetics 8 (1984), S. 323-326 
    Details
    ISSN: 1432-0983
    Keywords: Phytophthora infestans ; mtDNA ; Phatotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNA (mtDNA) of Phytophthora infestans has been isolated and preliminarily characterized. It has a low GC content of about 22.4% which is distinctly different from that of nuclear DNA (51 %). This property has been used to separate both DNA species in the presence of 4′,6-diamidine-2-phenylindole (DAPI) in CsCl density gradients. The use of cetyl triammonium bromide (CTAB) for extraction of DNA significantly reduced its degradation. The base distribution of the mtDNA shows a limited intramolecular heterogeneity. The molecule contains 36.2 ± 0.3 kb as revealed by endonuclease digestion and seems to be circular as shown by restriction mapping. No differences were found in restriction patterns between mtDNAs from various pathotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419731
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  • 2
    Electronic Resource
    Electronic Resource
    Repression of ant synthesis early in the lytic cycle of phage P22 (1981)
    Springer
    Molecular genetics and genomics 181 (1981), S. 74-81 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using SDS-polyacrylamide gel electrophoresis to study the early expression of P22 genes we show that early expression of the ant-gene (imm I region) is turned off after 6–8 min, independent of the ‘late’ acting mnt-repressor. A semi-clear mutant called cir5 is defective for this early ant turn-off. The mutation cir5 maps in the imm I region of P22 between genes mnt and ant. P22 cir5 mutants are defective for a repressor which acts in trans to regulate early ant synthesis. There appears to be no absolute requirement of the cir5 allele for the establishment of lysogeny. The overproduction of ant in the P22 cir5 mutant leads to a marked increase in abortive infections, killing the infected cells. The cir5-phenotype can be suppressed by an ant - mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339008
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  • 3
    Electronic Resource
    Electronic Resource
    Kinetics of c2-repressor synthesis in a regulatory defective P22 mutant (1981)
    Springer
    Molecular genetics and genomics 184 (1981), S. 147-150 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Phage P22 defective in gene 24 and harbouring the oc mutation k5 in OR exhibits a strongly increased c2-repressor synthesis after infection of non-lysogenic S. typhimurium. The repressor synthesis depends strictly on an intact c1 gene. The kinetics of its synthesis, as monitored by polyacrylamid gel electrophoresis, is the same as with P22 c +, namely a turn off 8–10 min after infection. — After infection of P22-lysogenic bacteria with either P22 24 − k5 or P22 24 − k5 cl, much lower amounts of repressor are synthesized but again with the same kinetics. These results suggest a cro-like function acting at PRE and PRM of P22. The possible reason for the c2 overproduction is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271211
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  • 4
    Electronic Resource
    Electronic Resource
    Kinetics of P22 early gene expression suggests a cro-like regulatory function (1981)
    Springer
    Molecular genetics and genomics 184 (1981), S. 151-157 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Analysis of phage-specified protein synthesis after phage infection of UV-irradiated cells shows a turn-off of early gene expression, a regulatory event that is independent of the known P22 regulatory functions. This supports the suggestion of a croλ-like function in P22. We have identified the products of genes 18 and int as contributing to the complex 40,000 dalton band in our SDS-polyacrylamide gels. Both gene products appear to be subject to regulation by the cro-like function of P22. Proteins of 33,000, 29,000, 27,000, 25,000, and 24,000 MW, specified by as yet unidentified P22 genes of the early leftward operon, are regulated by the same function. Our data suggest that the cro-like function is expressed from the early rightward operon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271212
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  • 5
    Electronic Resource
    Electronic Resource
    P22 antirepressor protein prevents in vivo recA-dependent proteolysis of P22 repressor (1983)
    Springer
    Molecular genetics and genomics 190 (1983), S. 427-431 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method was developed to demonstrate recA-dependent P22-repressor breakdown in vivo by SDS-polyacrylamide electrophoresis of unfractionated extracts of phage-infected, lysogenic Salmonella typhimurium strains TA1530 rec + and TA1530 recA1 -. The antirepressor of P22 is not cleaved by recA protein. Under conditions of unregulated ant-overproduction (Harvey et al. 1981) antirepressor protects c2-repressor in vivo against proteolytic cleavage by recA protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331072
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  • 6
    Electronic Resource
    Electronic Resource
    The immI region of Salmonella phage P22 (1984)
    Springer
    Molecular genetics and genomics 198 (1984), S. 110-115 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects were studied of three clear plaque mutations of phage P22 (cir4-1, cir5-1 and cir6-1) on antirepressor synthesis. The mutant site cir4-1 has no influence on the expression of gene ant. The cir5-1 and cir6-1 mutations prevent the repression of early ant synthesis shortly after infection: P22 cir5-1 exhibits a strong ant-overproduction because it renders the cir5 repressor protein defective for turning off early ant expression (Harvey et al. 1981). P22 cir6-1 exhibits only a low level of constitutive ant synthesis insensitive to cir5-directed repression. Complementation experiments between P22 wild type or mutant in the cir5 or cir6 sites reveal the following phenotypes of the cir6-1 mutation: (1) The cir6-1 site behaves as a weak promotor site insensitive to cir5-directed repression of ant synthesis. (2) In the P22 cir5-1 cir6-1 double recombinant the cir6-1 site is cis dominant preventing ant overproduction as observed in simple P22 cir5-1 infections. (3) Some of the deficient phenotypes of P22 cir6-1 can be complemented by co-infecting P22 mutants carrying a cir6 + allele. These results are explained by the following model: The active cir5 repressor protein is a dimer (or oligomer) and the cir5-1 and cir6-1 mutations map in different domains influencing the repressing as well as the dimer forming activities of this protein. Which particular cir6-1 phenotype is observed depends on the experimental conditions employed, and on the promotor site (either p ANT or cir6-1) from which ant expression procedes. P22 cir5-1 antam19 in su -bacteria does not produce any traceable amount of ant amber protein fragments, and an antam19 allele in trans to ant + restrains synthesis of ant protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328709
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  • 7
    Electronic Resource
    Electronic Resource
    The immI region of Salmonella phage P22 (1984)
    Springer
    Molecular genetics and genomics 198 (1984), S. 105-109 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Salmonella phage mutants P22 cir4-1, P22 cir5-1 and P22 cir6-1 at 37°C form more or less clear plaques. The mutants complement each other and clear mutations of the immC region (Prell et al. 1983). The mutants exhibit a strongly reduced frequency of lysogenisation, but form stable prophages. The low frequency of lysogenisation of P22 cir5-1 and of P22 cir6-1 is suppressed by an additional ant - mutation. Similarly, about 50% of turbid plaque revertants of both mutants carry ant - suppressor mutations. This suggests interference by the cir5-1 and cir6-1 mutations with the expression of gene ant. In contrast, the cir4-1 mutation seems not to interfere with ant expression, the latent periods of P22 cir4-1 and P22 cir5-1 are reduced and extended, respectively. The geneticly related Salmonella phage L carries a gene able to complement P22 cir4-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328708
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  • 8
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    Isolation and characterization of mitochondrial DNA of the oomycetous fungus Phytophthora infestans (1984)
    Springer
    Details
    Publication Date: 1984-05-01
    Print ISSN: 0172-8083
    Electronic ISSN: 1432-0983
    Topics: Biology
    Published by Springer
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