ISSN:
1572-9931
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A line of mouse cells containing a methylated and inactive herpes simplex virus thymidine kinase(TK) gene was irradiated with ultraviolet (UV) light in an attempt to induce expression of the inactiveTK gene. UV irradiation was shown to be capable of inducing expression of the viralTK gene in a dose-dependent manner. Analysis of the methylation pattern of the viralTK gene indicated that the activeTK gene in three UV-induced TK+ cell lines was methylated to a lesser extent than was the inactive viralTK gene in the parental cells. Analysis of the copy number of the viralTK gene in parental and UV-induced cell lines also revealed that the viralTK gene was amplified 3- to 20-fold in three of four UV-induced TK+ cell lines tested.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01535228
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