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  • 1
    Electronic Resource
    Electronic Resource
    Protection of pollen germination from adverse temperatures: a possible role for proline (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 6 (1983), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract After germination, pollen grains of Lilium longiflorum became very sensitive to short periods of heat stress as shown by the greatly reduced germination percentages upon subsequent incubation at the optimal temperature. Addition of proline to the incubation medium made pollen more resistant to heat. It was demonstrated that in a short time a large amount of proline was taken up by the cell. Germination and metabolic functions were completely or partially protected from heat damage by proline treatment. As well, it was shown that proline treatment at least partially protected pollen grains from cold stress. These results suggest that the high proline concentrations found in pollen of many species may confer resistance to germinating pollen grains at unfavourable temperatures thereby enhancing the chances of successful fertilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1365-3040.ep11588117
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  • 2
    Electronic Resource
    Electronic Resource
    Qualitative changes in protein synthesis in germinating pollen of Lilium longiflorum after a heat shock (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 7 (1984), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Germination and protein synthesis of lily pollen subjected to a short heat shock after imbibition are strongly inhibited. The proteins synthesized after the heat shock were analysed by polyacrylamide gel electrophoresis. The patterns obtained from heat-treated pollen are strikingly different from those of control samples. The difference is nearly completely climinated by a high concentration of proline in the incubation medium. This proline effect correlates with the protection of pollen germination from high temperature by the amino acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1365-3040.ep11572397
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  • 3
    Electronic Resource
    Electronic Resource
    Development of flower buds in thin-layer cultures of floral stalk tissue from tobacco: Role of hormones in different stages (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 61 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The in vitro development of flower buds was studied on tissue explants of epidermis and subepidermal cortex from the flower stalks of Nicotiana tabacum L. cv. Samsun. The number of flower buds formed depended mainly on cytokinin concentration. Auxin acted as a modifier in a complex way. In early development, NAA at 1 μM decreased the number of buds initiated and delayed bud emergence. At a later stage, auxin promoted bud outgrowth at the same concentration. Optimal results were obtained when explants were first incubated at low auxin concentration for 3–5 days and subsequently transferred to an elevated auxin level. Physiological processes that lead to flower bud initiation start very soon after the onset of incubation. This was inferred from experiments whereby explants were first cultured at an inductive cytokinin concentration and then transferred to a non-inductive hormone level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb06109.x
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  • 4
    Electronic Resource
    Electronic Resource
    Protein synthesis in germinating pollen of Petunia: role of proline (1982)
    Springer
    Planta 154 (1982), S. 199-203 
    Details
    ISSN: 1432-2048
    Keywords: Germination (pollen) ; Petunia ; Pollen ; Proline ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pollen of Petunia hybrida was germinated in artificial medium. At the beginning of the incubation, a large amount of proline, which comprises about half of the total free amino acid pool, was released into the medium. Part of this proline is reutilized by the pollen. Uptake of radioactive amino acids and their incorporation into proteins were studied. The highest rate of protein synthesis was found directly after the onset of germination. The endogenous free proline pool was found to be compartmentalized; one of the compartments is the protein precursor pool; its size is probably much less than 50% of the total free proline in the pollen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387864
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  • 5
    Electronic Resource
    Electronic Resource
    Proline metabolism in pollen: degradation of proline during germination and early tube growth (1983)
    Springer
    Planta 159 (1983), S. 46-49 
    Details
    ISSN: 1432-2048
    Keywords: Citrate cycle (proline) ; Petunia ; Pollen germination ; Proline degradation ; Respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Proline degradation inPetunia pollen germinated in vitro was studied in cultures supplemented with [14C]proline labeled at different positions. Despite its abundance in the cell, this amino acid is only a minor substrate for respiration. Proline is partially converted via the citrate cycle into metabolites which can be traced in the ethanol-insoluble fraction of the cellular constituents. The fact that this conversion is much more extensive than the use of proline in respiration indicates that, in germinating pollen, the citrate cycle serves mainly for synthetic purposes. There are no indications that proline carbon is used for feeding of other amino-acid pools used for protein synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00998813
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  • 6
    Electronic Resource
    Electronic Resource
    Cell-wall proteins in pollen and roots of Lilium longiflorum: extraction and partial characterization (1983)
    Springer
    Planta 158 (1983), S. 422-427 
    Details
    ISSN: 1432-2048
    Keywords: Cell wall, proteins ; Glycoprotein (cell wall) ; Hydroxyproline-containing protein (cell wall) ; Lilium ; Pollen ; Root (cell wall)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell-wall proteins of pollen grains, in-vitro-germinated pollen and young roots of Lilium longiflorum were studied by gel electrophoresis and amino-acid analysis. The proteins were removed from extensively purified walls by successive saline and alkali extractions. The major part including the hydroxyproline-containing proteins is covalently bound to the wall. Clear differences were observed between the proteins, especially the glycoproteins, of the pollen grain and the pollen tube. During elongation of the tubes some proteins decrease in quantity and many new proteins appear. The amount of protein in the cell walls is much lower in roots than in pollen and the root cell walls also contain fewer glycoproteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397735
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  • 7
    Electronic Resource
    Electronic Resource
    Changes in the adenylate energy charge and the induction of sporulation in Saccharomyces diastaticus (1981)
    Springer
    Archives of microbiology 129 (1981), S. 47-48 
    Details
    ISSN: 1432-072X
    Keywords: Adenylate energy charge ; Phosphate ; Saccharomyces ; Sporulation ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The induction of sporulation in yeast is generally accompanied by a sharp increase in energy metabolism which is evidenced by a rise of the adenylate energy charge by that time. The energy charge can be held at a low level by limitation of the phosphate supply in the growth medium. Ascus formation remains unaffected by this treatment. This suggests that the rise in ATP production normally encountered during early sporulation is not essential for the initiation of sporulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417178
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  • 8
    Electronic Resource
    Electronic Resource
    Book reviews (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 306-306 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303900
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  • 9
    Electronic Resource
    Electronic Resource
    Book reviews (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 248-248 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251154
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  • 10
    Electronic Resource
    Electronic Resource
    Book reviews (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 106-106 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253878
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