ISSN:
1432-0983
Schlagwort(e):
Chlamydomonas
;
Chloroplast
;
Organelle genetics
;
Streptomycin dependence
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary In the green alga Chlamydomonas reinhardtii, reciprocal crosses between strains carrying non-allelic chloroplast mutations to streptomycin dependence (sd-u) produce streptomycin sensitive (sd-u +) recombinant progeny. Transfer of these sd-u +progeny to streptomycin-containing medium results in a much higher frequency of recovery of streptomycin dependent isolates than expected by mutation. Failure to recover the more commonly encountered class of streptomycin resistant mutants also suggests that mutation is not responsible for appearance of the new dependent isolates. Backcrosses of these new sd-u isolates to strains carrying the original sd-u mutations demonstrate their allelism with the sd-u mutation contributed by the mt +parent. Earlier work by Schimmer and Arnold (1969, 1970a-d) indicated that newly isolated sensitive revertants of the streptomycin dependent mutant sd-u-3-18 also yielded high frequencies of sd-u cells but these were never analyzed genetically. We have now obtained new sd-u. isolates from streptomycin sensitive revertants of sd-u-318 and shown them all to be allelic with the original sd-u3-18 mutation. Thus “hidden” sd-u alleles can coexist with sd-u +alleles in heteroplasmic cells. These heteroplasmic cells are streptomycin sensitive in phenotype and may arise in crosses or from new mutation.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00420628
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