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  • Natural hybrids  (2)
  • Mycobacterium smegmatis  (1)
  • Phage DNA injection  (1)
  • 1980-1984  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Requirement for calcium ions in mycobacteriophage I3 DNA injection and propagation (1980)
    Springer
    Archives of microbiology 124 (1980), S. 249-254 
    Details
    ISSN: 1432-072X
    Keywords: Calcium ions ; Phage propagation ; Phage DNA injection ; Mycobacteriophage 13
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ca2+ ions are absolutely necessary for the propagation of mycobacteriophage I3 in synthetic medium. These ions are required for successful infection of the host and during the entire span of the intracellular development of the phage. A direct assay of the phage DNA injection using 32[P] labelled phage, showns that Ca2+ ions are necessary for the injection process. The injection itself is a slow process and takes 15 min to complete at 37°C. The bacteria infected in presence of Ca2+ tend to abort if the ions are subsequently withdrawn from the growth medium. The effect of calcium withdrawal is maximally felt during the early part of the latent period; however, later supplementation of Ca2+ ions salvage phage production and the mature phage progeny appear after a delayed interval, proportional to the time of addition of Ca2+.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427734
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  • 2
    Electronic Resource
    Electronic Resource
    Transfection of Mycobacterium smegmatis SN2 with mycobacteriophage I3 DNA (1983)
    Springer
    Archives of microbiology 136 (1983), S. 275-280 
    Details
    ISSN: 1432-072X
    Keywords: Transfection ; Mycobacterium smegmatis ; Mycobacteriophage I3 ; Calcium sensitization ; Glycine sensitization ; Protamine sulfate ; Exogenous RNA ; DNA uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mycobacterium smegmatis SN2 does not exhibit natural competence for the uptake of phage I3 DNA. Competence can artificially be induced by treatment with glycine or CaCl2, and the combination of both is even more effective. The efficiency of transfection can be improved by inclusion of protamine sulphate and heterologous RNA in the system. From 32P DNA uptake studies the major barrier for the entry of DNA has been found to be the complex cell wall. The efficiency of transfection calculated on the basis of fraction of DNA which has entered the cell is comparable to that of other bacterial systems. The phage development takes a longer time (7 h for one cycle) after transfection, as compared to infection (4 h).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425216
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  • 3
    Electronic Resource
    Electronic Resource
    Valency. II. Applications to molecules with first-row atoms (1983)
    Springer
    Theoretical chemistry accounts 63 (1983), S. 511-527 
    Details
    ISSN: 1432-2234
    Keywords: Valency of first-row atoms ; Free valence ; Bond indices ; Natural hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A quantum chemical definition of valency proposed in Part I is used to calculate the valency of carbon, nitrogen, oxygen, lithium, beryllium and boron in a number of compounds with the SINDO1 method. It is demonstrated that consistency of the basis set is necessary for comparable results. The general features of valency and bonding in these molecules are discussed. The π-electron concept of free valence is generalised to sigma systems and atoms in molecules are classified as subvalent, normal or hypervalent. The relation between valency and natural hybrid occupancy is illustrated. The symmetry properties of natural hybrid orbitals are discussed by means of group theory. A preliminary attempt is made to relate covalency and covalent reactivity. Bond indices and the σ, π character of bonds are obtained by a suitable partitioning and projection of valency into bonding and antibonding contributions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552653
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  • 4
    Electronic Resource
    Electronic Resource
    Valency. II. Applications to molecules with first-row atoms (1983)
    Springer
    Theoretical chemistry accounts 63 (1983), S. 511-527 
    Details
    ISSN: 1432-2234
    Keywords: Valency of first-row atoms ; Free valence ; Bond indices ; Natural hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A quantum chemical definition of valency proposed in Part I is used to calculate the valency of carbon, nitrogen, oxygen, lithium, beryllium and boron in a number of compounds with the SINDO1 method. It is demonstrated that consistency of the basis set is necessary for comparable results. The general features of valency and bonding in these molecules are discussed. The π-electron concept of free valence is generalised to sigma systems and atoms in molecules are classified as subvalent, normal or hypervalent. The relation between valency and natural hybrid occupancy is illustrated. The symmetry properties of natural hybrid orbitals are discussed by means of group theory. A preliminary attempt is made to relate covalency and covalent reactivity. Bond indices and the σ, π character of bonds are obtained by a suitable partitioning and projection of valency into bonding and antibonding contributions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02394810
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