Publication Date:
1984-08-31
Description:
Endothelial cell growth factor (ECGF), an anionic polypeptide mitogen, binds to immobilized heparin. The interaction between the acidic polypeptide and the anionic carbohydrate suggests a mechanism that is independent of ion exchange. Monoclonal antibodies to purified bovine ECGF inhibited the biological activity of ECGF in crude preparations of bovine brain. These data indicate that ECGF is the principal mitogen for endothelial cells from bovine brain, that heparin affinity chromatography may be used to purify and concentrate ECGF, and that the affinity of ECGF for heparin may have structural and perhaps biological significance.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Maciag, T -- Mehlman, T -- Friesel, R -- Schreiber, A B -- 310765/PHS HHS/ -- 4807/PHS HHS/ -- New York, N.Y. -- Science. 1984 Aug 31;225(4665):932-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6382607" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Monoclonal
;
*Brain Chemistry
;
Cattle
;
Cell Division
;
Chromatography, Affinity
;
Endothelial Growth Factors
;
Endothelium/*cytology
;
Growth Substances/immunology/isolation & purification/*metabolism/pharmacology
;
Heparin/*metabolism
;
Ion Exchange
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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