ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
α2-Macroglobulin (α2M), epidermal growth factor (EGF), and vesicular stomatitis virus (VSV) each enter cultured fibroblasts by receptor-mediated endocytosis. The present study defines some basic ionic requirements in the cell culture medium which are necessary for the maximal rate of endocytosis of these three ligands. Na+ and HCO3- were both necessary for maximal endocytosis of 125I-α2M, 125I-EGF, and 35S-VSV at 37°C. The ion specificities for both the anion and cation requirements were established. The binding of 125I-α2M to its cellular receptors at 4°C was unaffected by the absence of Na+ and HCO3- in the culture medium. In addition, the absence of Na+ and HCO3- in the culture medium did not reduce cellular uptake of horseradish peroxidase by fluid phase endocytosis. Na+ and HCO3- may be general requirements in receptor-mediated endocytosis.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1041130302
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