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  • 1
    ISSN: 1573-4943
    Keywords: photoaffinity probes ; peptide-conjugates from native protein fragments ; benzophenone probes ; p-azidobenzoyl probes ; antibodies to protein fragments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The advantages of using amino-directed photoprobes to couple native fragments, obtained by enzymatic digestion with trypsin, to protein carriers to prepare peptide-conjugates is described. The following photoprobe reagents were investigated:N-hydroxysuccinimidylp-azidobenzoate,N-hydroxysuccinimidyl ester ofp-azidobenzoylglycine,N-hydroxysuccinimidylp-benzoylbenzoate, and pentachlorophenyl ester ofp-benzoylbenzoyl glycine or the symmetric anhydride ofp-benzoylbenzoylglycine. These reagents modify only the NH2-terminal amino group and/or COOH-terminal ε-amino group of lysine of the tryptic fragments. Since the photoprobe is inert until photolysis, the probe-modified native fragment can be readily purified by high-performance liquid chromatography before cross-linking to the carrier molecule. The benzophenone photoprobes were shown to give the highest incorporation of peptide onto the protein carrier.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The protein journal 3 (1984), S. 465-478 
    ISSN: 1573-4943
    Keywords: photoaffinity probes ; synthetic peptide-conjugates ; solid-phase peptide synthesis ; benzophenone ; antibodies to synthetic peptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A general synthetic strategy is described for the preparation of peptide-conjugates where the peptides contain the NH2 terminal, COOH terminal, or internal regions of the protein sequence. Glycoprotein D of herpes simplex virus type 1 is used as a representative protein. Ten-residue peptide fragments of the native sequence were synthesized using standard solid-phase methodology. Photoprobes stable to conditions of synthesis and HF cleavage were coupled directly to the protected-peptide resin during synthesis. This one-step procedure eliminates the potential modification of functional groups in the sequence of interest that can occur when using chemically labile bifunctional reagents. Since the photoprobe is inert until photolysis, the synthetic peptide-probe can be readily purified by high-performance liquid chromatography before cross-linking to the carrier molecule. The following photoprobe derivatives were investigated: thep-azidobenzoyl,p-nitrophenylalanyl, andp-benzoylbenzoyl groups. The benzophenone photoprobes were shown to give the highest incorporation of peptide-probe with the protein carrier over a wide range ofpH and solvent conditions. For solid-phase synthesis three benzophenone photoprobes can be used: benzoylbenzoic acid, benzoylbenzoylglycine, andN e-(4-benzoylbenzoyl)-N α-t-butyloxycarbonyl-lysine.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Annals of biomedical engineering 12 (1984), S. 55-62 
    ISSN: 1573-9686
    Keywords: Carotid stenosis ; Bruit analysis ; Sound spectral analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Bruit analysis (phonoangiography) has been performed for many years as a method of characterizing arterial disease. Time displays of arterial bruits, particularly at the carotid bifurcation, have been used in an attempt to quantitate arterial narrowing. Despite the generalization that longer bruits and bruits which look and sound higher in frequency are often associated with severe disease, prospective studies have shown no useful predictive value for qualitative phonoangiography. In marked contrast, spectral bruit analysis or quantitative phonoangiography has been quite accurate in predicting the location and extent of carotid stenosis, and in distinguishing intrinsic from transmitted bruits. With this method, the peak systolic portion of the bruit is subjected to fast Fourier transform analysis. The peak frequency, beyond which amplitude drops as frequency increases further, is directly related to the residual lumen diameter of the stenotic common or internal carotid artery. Several blinded trials of this method have given results accurate to within 1 mm of angiographic values in 83–93% of cases studied. When used in conjunction with duplex doppler ultrasound scanning, 95% accuracy in diagnosis of patients with and without bruits may be achieved. This completely noninvasive method deserves more widespread use and may also be applicable to other cardiovascular sounds.
    Type of Medium: Electronic Resource
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