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  • Bound NTPs  (1)
  • Mycobacterium smegmatis  (1)
  • 1980-1984  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Presence of nucleoside triphosphates and calcium associated with mycobacteriophage I3 (1981)
    Springer
    Archives of microbiology 130 (1981), S. 50-53 
    Details
    ISSN: 1432-072X
    Keywords: Bound NTPs ; Injection of DNA ; Phage bound Ca2+ ; Equilibrium dialysis ; Mycobacteriophage I3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The association of nucleoside triphosphate molecules and calcium ions with purified particles of mycobacteriophage I3 has been documented. The content of nucleoside triphosphate has been determined to be 118 molecules per phage particle by equilibrium dialysis against labelled ATP or 148 molecules per phage particle by the direct determination of labelled nucleoside triphosphate. The concentration of bound Ca2+ exhibited a high degree of variation between different batches, which may be due to the nonspecific binding of Ca2+ by the virus particles. However, the tightly bound Ca2+ not removable by dialysis against calciumspecific chelating agent, showed a constant value of 2985 atoms/phage particle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00527071
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  • 2
    Electronic Resource
    Electronic Resource
    Transfection of Mycobacterium smegmatis SN2 with mycobacteriophage I3 DNA (1983)
    Springer
    Archives of microbiology 136 (1983), S. 275-280 
    Details
    ISSN: 1432-072X
    Keywords: Transfection ; Mycobacterium smegmatis ; Mycobacteriophage I3 ; Calcium sensitization ; Glycine sensitization ; Protamine sulfate ; Exogenous RNA ; DNA uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mycobacterium smegmatis SN2 does not exhibit natural competence for the uptake of phage I3 DNA. Competence can artificially be induced by treatment with glycine or CaCl2, and the combination of both is even more effective. The efficiency of transfection can be improved by inclusion of protamine sulphate and heterologous RNA in the system. From 32P DNA uptake studies the major barrier for the entry of DNA has been found to be the complex cell wall. The efficiency of transfection calculated on the basis of fraction of DNA which has entered the cell is comparable to that of other bacterial systems. The phage development takes a longer time (7 h for one cycle) after transfection, as compared to infection (4 h).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425216
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    Paper (German National Licenses)
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